BRENDA - Enzyme Database show
show all sequences of 3.6.1.64

NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA and growth arrest

Iyama, T.; Abolhassani, N.; Tsuchimoto, D.; Nonaka, M.; Nakabeppu, Y.; Nucleic Acids Res. 38, 4834-4843 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.062
-
IDP
pH 7.5, 37°C
Homo sapiens
0.088
-
dIDP
pH 7.5, 37°C
Homo sapiens
0.32
-
dGDP
pH 7.5, 37°C
Homo sapiens
0.33
-
GDP
pH 7.5, 37°C
Homo sapiens
15.7
-
XDP
pH 7.5, 37°C
Homo sapiens
22.1
-
ITP
pH 7.5, 37°C
Homo sapiens
24.1
-
dITP
pH 7.5, 37°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleolus
-
Homo sapiens
5730
-
nucleolus
-
Mus musculus
5730
-
nucleus
-
Homo sapiens
5634
-
nucleus
-
Mus musculus
5634
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
maximum activity at 1 mM
Homo sapiens
additional information
divalent cation absolutely required. No IDP hydrolysis in the presence of 5mM Mn2+, Co2+ or Ca2+
Homo sapiens
Zn2+
may partly substitue for Mn2+
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Homo sapiens
Q96DE0
bifunctional U8 snoRNA-decapping enzyme and IDP phosphatase
-
Mus musculus
Q6P3D0
bifunctional U8 snoRNA-decapping enzyme and IDP phosphatase
-
Reaction
Reaction
Commentary
Organism
dIDP + H2O = dIMP + phosphate
(2)
Homo sapiens
IDP + H2O = IMP + phosphate
(1)
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
and lung, highest expression
Homo sapiens
-
lung
and kidney, highest expression
Homo sapiens
-
additional information
expression in all tissues examined
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dGDP + H2O
-
720583
Homo sapiens
dGMP + phosphate
-
-
-
?
dIDP + H2O
-
720583
Homo sapiens
dIMP + phosphate
-
-
-
?
dITP + H2O
-
720583
Mus musculus
dIMP + diphosphate
-
-
-
?
dITP + H2O
-
720583
Homo sapiens
dIMP + diphosphate
hydrolysis of ITP generates IMP but not phosphate
-
-
?
GDP + H2O
-
720583
Homo sapiens
GMP + phosphate
-
-
-
?
IDP + H2O
-
720583
Homo sapiens
IMP + phosphate
-
-
-
?
IDP + H2O
-
720583
Mus musculus
IMP + phosphate
-
-
-
?
ITP + H2O
-
720583
Homo sapiens
IDP + phosphate
-
-
-
?
XDP + H2O
-
720583
Homo sapiens
XMP + phosphate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
temperature-dependent increase in IDP hydrolyzing activity up to 60°C
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.008
-
dGDP
pH 7.5, 37°C
Homo sapiens
0.009
-
GDP
pH 7.5, 37°C
Homo sapiens
0.016
-
dIDP
pH 7.5, 37°C
Homo sapiens
0.016
-
IDP
pH 7.5, 37°C
Homo sapiens
0.043
-
XDP
pH 7.5, 37°C
Homo sapiens
0.051
-
ITP
pH 7.5, 37°C
Homo sapiens
0.053
-
dITP
pH 7.5, 37°C
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.062
-
IDP
pH 7.5, 37°C
Homo sapiens
0.088
-
dIDP
pH 7.5, 37°C
Homo sapiens
0.32
-
dGDP
pH 7.5, 37°C
Homo sapiens
0.33
-
GDP
pH 7.5, 37°C
Homo sapiens
15.7
-
XDP
pH 7.5, 37°C
Homo sapiens
22.1
-
ITP
pH 7.5, 37°C
Homo sapiens
24.1
-
dITP
pH 7.5, 37°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleolus
-
Homo sapiens
5730
-
nucleolus
-
Mus musculus
5730
-
nucleus
-
Homo sapiens
5634
-
nucleus
-
Mus musculus
5634
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
maximum activity at 1 mM
Homo sapiens
additional information
divalent cation absolutely required. No IDP hydrolysis in the presence of 5mM Mn2+, Co2+ or Ca2+
Homo sapiens
Zn2+
may partly substitue for Mn2+
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
and lung, highest expression
Homo sapiens
-
lung
and kidney, highest expression
Homo sapiens
-
additional information
expression in all tissues examined
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dGDP + H2O
-
720583
Homo sapiens
dGMP + phosphate
-
-
-
?
dIDP + H2O
-
720583
Homo sapiens
dIMP + phosphate
-
-
-
?
dITP + H2O
-
720583
Mus musculus
dIMP + diphosphate
-
-
-
?
dITP + H2O
-
720583
Homo sapiens
dIMP + diphosphate
hydrolysis of ITP generates IMP but not phosphate
-
-
?
GDP + H2O
-
720583
Homo sapiens
GMP + phosphate
-
-
-
?
IDP + H2O
-
720583
Homo sapiens
IMP + phosphate
-
-
-
?
IDP + H2O
-
720583
Mus musculus
IMP + phosphate
-
-
-
?
ITP + H2O
-
720583
Homo sapiens
IDP + phosphate
-
-
-
?
XDP + H2O
-
720583
Homo sapiens
XMP + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
temperature-dependent increase in IDP hydrolyzing activity up to 60°C
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.008
-
dGDP
pH 7.5, 37°C
Homo sapiens
0.009
-
GDP
pH 7.5, 37°C
Homo sapiens
0.016
-
dIDP
pH 7.5, 37°C
Homo sapiens
0.016
-
IDP
pH 7.5, 37°C
Homo sapiens
0.043
-
XDP
pH 7.5, 37°C
Homo sapiens
0.051
-
ITP
pH 7.5, 37°C
Homo sapiens
0.053
-
dITP
pH 7.5, 37°C
Homo sapiens
General Information
General Information
Commentary
Organism
physiological function
knockdown in HeLa MR cells causes cell cycle arrest in S-phase, reduces cell proliferation, and leads to increased accumulation of single-strand breaks in nuclear DNA as well as increased levels of inosine in RNA
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
knockdown in HeLa MR cells causes cell cycle arrest in S-phase, reduces cell proliferation, and leads to increased accumulation of single-strand breaks in nuclear DNA as well as increased levels of inosine in RNA
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0022
-
dITP
pH 7.5, 37°C
Homo sapiens
0.0023
-
ITP
pH 7.5, 37°C
Homo sapiens
0.0027
-
XDP
pH 7.5, 37°C
Homo sapiens
0.0257
-
dGDP
pH 7.5, 37°C
Homo sapiens
0.0261
-
GDP
pH 7.5, 37°C
Homo sapiens
0.183
-
dIDP
pH 7.5, 37°C
Homo sapiens
0.251
-
IDP
pH 7.5, 37°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0022
-
dITP
pH 7.5, 37°C
Homo sapiens
0.0023
-
ITP
pH 7.5, 37°C
Homo sapiens
0.0027
-
XDP
pH 7.5, 37°C
Homo sapiens
0.0257
-
dGDP
pH 7.5, 37°C
Homo sapiens
0.0261
-
GDP
pH 7.5, 37°C
Homo sapiens
0.183
-
dIDP
pH 7.5, 37°C
Homo sapiens
0.251
-
IDP
pH 7.5, 37°C
Homo sapiens
Other publictions for EC 3.6.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735030
Tresaugues
Structural basis for the speci ...
Homo sapiens
PLoS ONE
10
e0131507
2015
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720583
Iyama
NUDT16 is a (deoxy)inosine dip ...
Homo sapiens, Mus musculus
Nucleic Acids Res.
38
4834-4843
2010
-
-
1
-
-
-
-
7
4
3
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4
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2
-
3
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9
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1
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7
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1
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7
4
3
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3
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9
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7
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1
1
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7
7