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Literature summary for 3.6.1.62 extracted from

  • Lu, G.; Zhang, J.; Li, Y.; Li, Z.; Zhang, N.; Xu, X.; Wang, T.; Guan, Z.; Gao, G.F.; Yan, J.
    hNUDT16: a universal decapping enzyme for small nucleolar RNA and cytoplasmic mRNA (2011), Protein Cell, 2, 64-73.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.62

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E76Q mutation totally abolishes the decapping activity under the standard assaying condition Homo sapiens
E79Q mutation decreases the decapping activity of the enzyme Homo sapiens
E80Q mutation totally abolishes the decapping activity under the standard assaying condition Homo sapiens
R75L the mutant displays a weaker capability of hydrolysis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ confer only limited hydrolytic capability on hNUDT16. After 30 min incubation of the RNA substrate with the enzyme, only tiny amounts of m7GDP product are observed on the migration profiles Homo sapiens
Mg2+ hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs. hNUDT16 without the coordination of metals can not catalyze the hydrolytic reaction since no detectable cleaved products can be observed for either the U8 snoRNA or the mRNA substrate. Both Mg2+ and Mn2+ can effectively switch the protein from apoenzyme to holoenzyme. Mn2+ is more efficient as the activating factor Homo sapiens
Mn2+ hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs. hNUDT16 without the coordination of metals can not catalyze the hydrolytic reaction since no detectable cleaved products can be observed for either the U8 snoRNA or the mRNA substrate. Both Mg2+ and Mn2+ can effectively switch the protein from apoenzyme to holoenzyme. Mn2+ is more efficient as the activating factor Homo sapiens
Zn2+ confer only limited hydrolytic capability on hNUDT16. After 30 min incubation of the RNA substrate with the enzyme, only tiny amounts of m7GDP product are observed on the migration profiles Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
m7G5'ppp5'-mRNA + H2O Homo sapiens hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs m7GDP + 5'-phospho-mRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-(N7-methylguanosine 5'-triphospho)-mRNA + H2O luciferase mRNA Homo sapiens m7GDP + 5'-phospho-mRNA
-
 ?
m7G5'ppp5'-mRNA + H2O hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs Homo sapiens m7GDP + 5'-phospho-mRNA
-
 ?
m7G5'ppp5'-U8 snomRNA + H2O hNUDT16 relies on divalent cations for its cap-hydrolysis activity to remove m7GDP and m227GDP from RNAs. hNUDT16 without the coordination of metals can not catalyze the hydrolytic reaction since no detectable cleaved products can be observed for either the U8 snoRNA or the mRNA substrate. Both Mg2+ and Mn2+ can effectively switch the protein from apoenzyme to holoenzyme. Mn2+ is more efficient as the activating factor Homo sapiens m7GDP + 5'-phospho-U8 snomRNA
-
 ?

Synonyms

Synonyms Comment Organism
hNUDT16
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens