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Literature summary for 3.6.1.62 extracted from

  • Parrish, S.; Resch, W.; Moss, B.
    Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression (2007), Proc. Natl. Acad. Sci. USA, 104, 2139-2144.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.62

Cloned(Commentary)

Cloned (Comment) Organism
(MBP)-D10 fusion is synthesized in Escherichia coli Vaccinia virus

Protein Variants

Protein Variants Comment Organism
E141Q inactive mutant enzyme Vaccinia virus
E144Q/E145Q inactive mutant enzyme Vaccinia virus

Inhibitors

Inhibitors Comment Organism Structure
m7G5'ppp5'G inhibits decapping at large molar ratios relative to capped RNA substrate Vaccinia virus
m7GDP inhibits decapping at large molar ratios relative to capped RNA substrate Vaccinia virus
m7GTP inhibits decapping at large molar ratios relative to capped RNA substrate Vaccinia virus
additional information even higher concentrations of unmethylated analogs do not inhibit Vaccinia virus
uncapped RNA high concentrations of uncapped RNA are inhibitory Vaccinia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 m7G5'ppp5'-mRNA pH 7.5, 37°C Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
m7G5'ppp5'-mRNA + H2O Vaccinia virus the mRNA decapping activity of D10 allows vaccinia virus to accelerate degradation of viral and host messages. By degrading host mRNAs, VACV D10 may have an immunomodulatory role m7GDP + 5'-phospho-mRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant D10 fusion protein Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
m7G5'ppp5'-mRNA + H2O the mRNA decapping activity of D10 allows vaccinia virus to accelerate degradation of viral and host messages. By degrading host mRNAs, VACV D10 may have an immunomodulatory role Vaccinia virus m7GDP + 5'-phospho-mRNA
-
 ?
m7G5'ppp5'-mRNA + H2O RNAs of 24-309 nucleotides are decapped to comparable extents, whereas the cap of a 12-nt RNA is uncleaved. D10 recognizes its substrate through interaction with both cap and RNA moieties. The Nudix/MutT motif of VACV D10 is essential for RNA cap cleavage Vaccinia virus m7GDP + 5'-phospho-mRNA
-
 ?

Synonyms

Synonyms Comment Organism
D10 protein
-
 Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Vaccinia virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.003
-
 pH 7.5, 37°C Vaccinia virus m7GTP
0.003
-
 pH 7.5, 37°C Vaccinia virus uncapped RNA
0.063
-
 pH 7.5, 37°C Vaccinia virus m7G5'ppp5'G
0.3
-
 pH 7.5, 37°C Vaccinia virus m7GDP

General Information

General Information Comment Organism
physiological function the mRNA decapping activity of D10 allows Vaccinia virus to accelerate degradation of viral and host messages. By degrading host mRNAs, VACV D10 may have an immunomodulatory role. Although it may seem self-destructive for a virus to encode a decapping and a capping enzyme, accelerated mRNA turnover helps eliminate competing host mRNAs and allows stagespecific synthesis of viral proteins Vaccinia virus