Cloned (Comment) | Organism |
---|---|
- |
Vaccinia virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | decapping actiovity is abolished by point mutations in the Nudix hydrolase motif | Vaccinia virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
m7G5'ppp5'G | D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA | Vaccinia virus | |
N7-methylguanosine 5'-diphosphate | D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA | Vaccinia virus | |
N7-methylguanosine 5'-triphosphate | D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA | Vaccinia virus | |
uncapped RNA | D9 differs from D10 in having greater sensitivity to inhibition by uncapped RNA | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
m7G5'ppp5'-mRNA + H2O | Vaccinia virus | D9 is expressed early in infection and D10 late. It is suggested that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner | m7GDP + 5'-phospho-mRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
m7G5'ppp5'-mRNA + H2O | D9 is expressed early in infection and D10 late. It is suggested that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner | Vaccinia virus | m7GDP + 5'-phospho-mRNA | - |
? | |
m7G5'ppp5'-mRNA + H2O | decapping is specific for a methylated cap attached to RNA. D9 differs from D10 in requiring a longer capped RNA substrate for optimal activity. Only trace amounts of the 12- and 24-nt RNA substrates are cleaved. Higher activity with 36-, 48-, or 309-nt RNA substrates | Vaccinia virus | m7GDP + 5'-phospho-mRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D9 protein | - |
Vaccinia virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Vaccinia virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Vaccinia virus |
General Information | Comment | Organism |
---|---|---|
physiological function | D9 is expressed early in infection and D10 late. It is suggested that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner | Vaccinia virus |