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Literature summary for 3.6.1.62 extracted from

  • Soulière, M.F.; Perreault, J.P.; Bisaillon. M.
    Characterization of the vaccinia virus D10 decapping enzyme provides evidence for a two-metal-ion mechanism (2009), Biochem. J., 420, 27-35.
    View publication on PubMed
Search for more literature for 3.6.1.62

Protein Variants

Protein Variants Comment Organism
E132A 7.8% of wild-type activity. The E132A mutant shows a 5fold reduced affinity for Mg2+, but retains a Mn2+ affinity similar to that of the wild type Vaccinia virus
E141A 10% of wild-type activity. The E141A mutant demonstrates only slight variations in both Mg2+ and Mn2+ binding Vaccinia virus
E145A 12.2% of wild-type activity. the E145A mutant shows 2-fold reduced affinity for Mg2+ and 6-fold reduced Mn2+ binding Vaccinia virus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ supports D10p catalytic activity, but fails to demonstrate a synergistic effect on the enzyme when tested with Mn2+ ions Vaccinia virus
Mg2+ presence of two metal-ion-binding sites on the enzyme. Synergistic activation of the enzyme in the presence of Mg2+ and Mn2+ ions, suggesting the existence of two metal-ion binding sites on the D10 protein. One metal ion is co-ordinated by Glu132, while the second metal ion is co-ordinated by Glu145 Vaccinia virus
Mn2+ presence of two metal-ion-binding sites on the enzyme. Synergistic activation of the enzyme in the presence of Mg2+ and Mn2+ ions, suggesting the existence of two metal-ion binding sites on the D10 protein. One metal ion is co-ordinated by Glu132, while the second metal ion is co-ordinated by Glu145 Vaccinia virus
additional information Ca2+ ions can not support D10p activity and does not produce a synergistic activation ofD10p in the presence of Mn2+ Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
m7G5'ppp5'-mRNA + H2O Vaccinia virus
-
 m7GDP + 5'-phospho-mRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
m7G5'ppp5'-mRNA + H2O
-
 Vaccinia virus m7GDP + 5'-phospho-mRNA
-
 ?
m7G5'ppp5'-mRNA + H2O cleavage between the alpha- and beta-phosphates of the m7GpppN cap Vaccinia virus m7GDP + 5'-phospho-mRNA
-
 ?

Synonyms

Synonyms Comment Organism
D10 decapping enzyme
-
 Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Vaccinia virus