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Literature summary for 3.6.1.6 extracted from

  • Kawakita, N.; Yamazaki, M.
    Allosteric properties of nucleoside diphosphatase. Activation by pyridoxal 5'-phosphate and specific modification of effector binding sites (1978), Biochem. J., 17, 3546-3551.
    View publication on PubMed
Search for more literature for 3.6.1.6

Activating Compound

Activating Compound Comment Organism Structure
ATP activates, no stimulation of the reaction which is activated by pyridoxal 5'-phosphate Rattus norvegicus
pyridoxal 5'-phosphate activation due to Schiff base formation with epsilon-amino groups of Lys residues of the enzyme, Km: 0.0052 mM, no further stimulation of the reaction which is maximally activated by pyridoxal 5'-phosphate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 IDP native enzyme in presence of ATP Rattus norvegicus
2.94
-
 IDP native enzyme in absesence of ATP Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 2 * 63000, SDS-PAGE Rattus norvegicus
130000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
358.1
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
IDP + H2O
-
 Rattus norvegicus IMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 7.4, 50 mM triethanolamine hydrochloride, about 80% loss of activity after 16 min, inactivation is decreased by 2 mM pyridoxal phosphate Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 10 min, about 40% loss of activity of the native enzyme, about 30% loss of activity of the enzyme after treatment with pyridoxal phosphate and reduction with NaBH4 Rattus norvegicus
6
-
 10 min, native enzyme and enzyme after treatment with pyridoxal phosphate and reduction with NaBH4, stable Rattus norvegicus
9
-
 10 min, about 25% loss of activity of the native enzyme, about 5% loss of activity of the enzyme after treatment with pyridoxal phosphate and reduction with NaBH4 Rattus norvegicus