Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 1 mM, 27% inhibition of thiamin-diphosphatase activity, competitive | Rattus norvegicus | |
AMP | slight competitive inhibition of thiamin-diphosphatase activity | Rattus norvegicus | |
ATP | slight competitive inhibition of thiamin-diphosphatase activity | Rattus norvegicus | |
chlorpromazine | 1 mM, 15% inhibition | Rattus norvegicus | |
diphosphate | complete inhibition at 1 mM, 61% inhibition at 0.06 mM | Rattus norvegicus | |
F- | 100 mM KF, 33% inhibition | Rattus norvegicus | |
IMP | slight competitive inhibition of thiamin-diphosphatase activity | Rattus norvegicus | |
NEM | 1 mM, 16% inhibition | Rattus norvegicus | |
Promethazine | 1 mM, 15% inhibition | Rattus norvegicus | |
pyridoxal 5'-phosphate | 1 mM, 43% inhibition of thiamin-diphosphatase activity, competitive | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
GDP | in presence of Mn2+ | Rattus norvegicus | |
0.54 | - |
IDP | in presence of Mn2+ | Rattus norvegicus | |
0.66 | - |
thiamine diphosphate | in presence of Mn2+ | Rattus norvegicus | |
1.06 | - |
UDP | in presence of Mn2+ | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: GDP, TPP, IDP, UDP, CDP | Rattus norvegicus | |
Cd2+ | Cd2+ can partially replace Mn2+, 16%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Cd2+, 38% | Rattus norvegicus | |
Co2+ | Co2+ can partially replace Mn2+, 20%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Co2+, 55% | Rattus norvegicus | |
Manganese | metalloenzyme contains 0.9 mol zinc and 0.1 mol manganese per mol of 65000 Da subunit | Rattus norvegicus | |
Mg2+ | in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: GDP, IDP, TPP, UDP, CDP | Rattus norvegicus | |
Mn2+ | in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: TPP, IDP, GDP, UDP, CDP, dTDP | Rattus norvegicus | |
Zinc | metalloenzyme contains 0.9 mol zinc and 0.1 mol manganese per mol of 65000 Da subunit | Rattus norvegicus | |
Zn2+ | Zn2+ can partially replace Mn2+, 4%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Zn2+, 23% | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
1 * 75000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.43 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | no activity | Rattus norvegicus | AMP + phosphate | - |
? | |
GDP + H2O | - |
Rattus norvegicus | GMP + phosphate | - |
? | |
IDP + H2O | - |
Rattus norvegicus | IMP + phosphate | - |
? | |
TPP + H2O | is hydrolyzed twice as efficiently as nucleoside diphosphates | Rattus norvegicus | thiamine phosphate + phosphate | - |
? | |
UDP + H2O | - |
Rattus norvegicus | UMP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
hydrolysis of GDP in presence of Mg2+, TTPase activity in presence of Mg2+ | Rattus norvegicus |
7 | - |
GDPase activity in presence of Mn2+ and Ca2+ | Rattus norvegicus |
7.4 | - |
hydrolysis of thiamin-diphosphate in presence of Mn2+ | Rattus norvegicus |
8 | 8.5 | TTPase activity in presence of Ca2+ | Rattus norvegicus |