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Literature summary for 3.6.1.57 extracted from

  • Rangarajan, E.S.; Proteau, A.; Cui, Q.; Logan, S.M.; Potetinova, Z.; Whitfield, D.; Purisima, E.O.; Cygler, M.; Matte, A.; Sulea, T.; Schoenhofen, I.C.
    Structural and functional analysis of Campylobacter jejuni PseG: a udp-sugar hydrolase from the pseudaminic acid biosynthetic pathway (2009), J. Biol. Chem., 284, 20989-21000.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.57

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of Campylobacter jejuni PseG in apo-form and as a complex with its UDP product at 1.8 and 1.85 A resolution. Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
H17F almost complete inactivation Campylobacter jejuni
H17L almost complete inactivation Campylobacter jejuni
H17N kcat/KM for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose is 9.4% of the wild-type value Campylobacter jejuni
N255A kcat/KM for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose is 0.64% of the wild-type value Campylobacter jejuni
Y78F kcat/KM for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose is 2.1% of the wild-type value Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, wild-type enzym Campylobacter jejuni
0.44
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme N255A Campylobacter jejuni
0.88
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme Y78F Campylobacter jejuni
1.3
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme H17N Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32380
-
 His6-tagged enzyme, electrospray ionization mass spectrometry Campylobacter jejuni
32383
-
 1 * 32383, calculated from sequence Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O Campylobacter jejuni the enzyme is involved in biosynthesis of pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid). Flagella of Campylobacter jejuni are important virulence determinants, whose proper assembly and function are dependent upon glycosylation at multiple positions by sialic acid-like sugars, such as 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid (pseudaminic acid) 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q0P8U5
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information molecular modeling identifies a His17-coordinated water molecule as the putative nucleophile and suggests the UDP-sugar substrate adopts a twist-boat conformation upon binding to PseG, enhancing the exposure of the anomeric bond cleaved and favoring inversion at C1 Campylobacter jejuni ?
-
 ?
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O the enzyme is involved in biosynthesis of pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid). Flagella of Campylobacter jejuni are important virulence determinants, whose proper assembly and function are dependent upon glycosylation at multiple positions by sialic acid-like sugars, such as 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid (pseudaminic acid) Campylobacter jejuni 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP
-
 ?
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O His17 functions as an active site base, thereby activating the nucleophilic water molecule for attack of the anomeric C-O bond of the UDP-sugar. No activity was observed with UDP-4-amino-4,6-dideoxy-beta-L-AltNAc or UDP-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucopyranose or UDP-2,4-diacetamido-bacillosamine Campylobacter jejuni 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32383, calculated from sequence Campylobacter jejuni

Synonyms

Synonyms Comment Organism
PseG
-
 Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme N255A Campylobacter jejuni
2.8
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme H17N Campylobacter jejuni
8.3
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme Y78F Campylobacter jejuni
25
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, wild-type enzym Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Campylobacter jejuni

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid). Flagella of Campylobacter jejuni are important virulence determinants, whose proper assembly and function are dependent upon glycosylation at multiple positions by sialic acid-like sugars, such as pseudaminic acid (i.e. 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-nonulosonic acid) Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme N255A Campylobacter jejuni
2.1
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme H17N Campylobacter jejuni
9.4
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, mutant enzyme Y78F Campylobacter jejuni
100
-
 UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose pH 7.3, 37°C, wild-type enzym Campylobacter jejuni