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Literature summary for 3.6.1.56 extracted from

  • Yakushiji, H.; Maraboeuf, F.; Takahashi, M.; Deng, Z.S.; Kawabata, S.; Nakabeppu, Y.; Sekiguchi, M.
    Biochemical and physicochemical characterization of normal and variant forms of human MTH1 protein with antimutagenic activity (1997), Mutat. Res., 384, 181-194.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
both types of MTH1 protein (Met83-MTH1 and Val83-MTH1) are expressed in Escherichia coli mutT- cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
V83M Met83-MTH1 is more thermolabile than that of Val83-MTH1. Substitution of valine for methionine at the residue 83 of MTH1 protein appears to lead to alteration in the secondary structure which renders the protein more labile than the normal type protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8-oxo-dGTP + H2O Homo sapiens 8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate is produced during cellular metabolism, and its misincorporation into DNA causes mutation. MTH1 hydrolyzes 8-oxo-dGTP to the corresponding nucleoside monophosphate, thereby preventing misincorporation of 8-oxo-7,8-dihydroguanine into DNA. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP 8-oxo-dGMP + diphosphate
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Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-oxo-dGTP + H2O 8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate is produced during cellular metabolism, and its misincorporation into DNA causes mutation. MTH1 hydrolyzes 8-oxo-dGTP to the corresponding nucleoside monophosphate, thereby preventing misincorporation of 8-oxo-7,8-dihydroguanine into DNA. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-dGMP + diphosphate
-
?
8-oxo-dGTP + H2O 8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-dGMP + diphosphate
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?

Synonyms

Synonyms Comment Organism
MTH1
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Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, both types of MTH1 protein (Met83-MTH1 and Val83-MTH1) retain full activity until the temperature reaches 55°C Homo sapiens
60
-
5 min, Met83-MTH1 loses 31-46% of its activity, Val83-MTH1 retains full activity Homo sapiens