Cloned (Comment) | Organism |
---|---|
both types of MTH1 protein (Met83-MTH1 and Val83-MTH1) are expressed in Escherichia coli mutT- cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V83M | Met83-MTH1 is more thermolabile than that of Val83-MTH1. Substitution of valine for methionine at the residue 83 of MTH1 protein appears to lead to alteration in the secondary structure which renders the protein more labile than the normal type protein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-oxo-dGTP + H2O | Homo sapiens | 8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate is produced during cellular metabolism, and its misincorporation into DNA causes mutation. MTH1 hydrolyzes 8-oxo-dGTP to the corresponding nucleoside monophosphate, thereby preventing misincorporation of 8-oxo-7,8-dihydroguanine into DNA. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | 8-oxo-dGMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-oxo-dGTP + H2O | 8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate is produced during cellular metabolism, and its misincorporation into DNA causes mutation. MTH1 hydrolyzes 8-oxo-dGTP to the corresponding nucleoside monophosphate, thereby preventing misincorporation of 8-oxo-7,8-dihydroguanine into DNA. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | Homo sapiens | 8-oxo-dGMP + diphosphate | - |
? | |
8-oxo-dGTP + H2O | 8-oxo-dGTP i.e. 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | Homo sapiens | 8-oxo-dGMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTH1 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, both types of MTH1 protein (Met83-MTH1 and Val83-MTH1) retain full activity until the temperature reaches 55°C | Homo sapiens |
60 | - |
5 min, Met83-MTH1 loses 31-46% of its activity, Val83-MTH1 retains full activity | Homo sapiens |