Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-R3-pRARE2 cells | Bartonella henselae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 10% (w/v) PEG 4000, 0.1 M MgCl2 | Bartonella henselae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme binds catalytically essential Mg2+ | Bartonella henselae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bartonella henselae | A0A0H3LW43 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel Sepharose column chromatography and Superdex 75 gel filtration | Bartonella henselae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydro-8-oxo-GTP + H2O | - |
Bartonella henselae | 7,8-dihydro-8-oxo-GMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MutT | - |
Bartonella henselae |
Nudix hydrolase | - |
Bartonella henselae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme irreversibly unfolds and precipitates out of solution upon heating, with a melting temperature of 60°C | Bartonella henselae |