Cloned (Comment) | Organism |
---|---|
lpxH overexpression is toxic to cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.01% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0617 | - |
UDP-N2,O3-bis((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine | pH 8.0, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | stimulates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26893 | - |
x * 26893, calculated from sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-2,3-bis((3R)-3-hydroxymyristoyl)alpha-D-glucosamine + H2O | Escherichia coli | step of lipid A biosynthesis | 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate + UMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P43341 | - |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.2 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with UDP-N-acetylglucosamine and UDP-3-O-(R)-(3-hydroxymyristoyl)glucosamine, LpxH does not cleave CDP-diacylglycerol | Escherichia coli | ? | - |
? | |
UDP-2,3-bis((3R)-3-hydroxymyristoyl)alpha-D-glucosamine + H2O | step of lipid A biosynthesis | Escherichia coli | 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate + UMP | - |
? | |
UDP-N2,O3-bis((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine + H2O | strict requirement for a diacylated substrate. LpxH hydrolysis incorporates H218O into UMP by catalyzing the attack of water on the alpha-phosphorus atom of UDP-2,3-diacylglucosamine | Escherichia coli | N2,O3-bis((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine 1-phosphate + UMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26893, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
LpxH | - |
Escherichia coli |
UDP-2,3-diacylglucosamine hydrolase | - |
Escherichia coli |
UDP-2,3-diacylglucosamine pyrophosphatase | - |
Escherichia coli |
YbbF | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 50% of maximal activity, pH 9.5: about 50% of maximal activity | Escherichia coli |