Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.54 extracted from

  • Babinski, K.J.; Ribeiro, A.A.; Raetz, C.R.
    The Escherichia coli gene encoding the UDP-2,3-diacylglucosamine pyrophosphatase of lipid A biosynthesis (2002), J. Biol. Chem., 277, 25937-2546.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
lpxH overexpression is toxic to cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 0.01% Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0617
-
UDP-N2,O3-bis((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine pH 8.0, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26893
-
x * 26893, calculated from sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-2,3-bis((3R)-3-hydroxymyristoyl)alpha-D-glucosamine + H2O Escherichia coli step of lipid A biosynthesis 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate + UMP
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P43341
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63.2
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with UDP-N-acetylglucosamine and UDP-3-O-(R)-(3-hydroxymyristoyl)glucosamine, LpxH does not cleave CDP-diacylglycerol Escherichia coli ?
-
?
UDP-2,3-bis((3R)-3-hydroxymyristoyl)alpha-D-glucosamine + H2O step of lipid A biosynthesis Escherichia coli 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate + UMP
-
?
UDP-N2,O3-bis((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine + H2O strict requirement for a diacylated substrate. LpxH hydrolysis incorporates H218O into UMP by catalyzing the attack of water on the alpha-phosphorus atom of UDP-2,3-diacylglucosamine Escherichia coli N2,O3-bis((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine 1-phosphate + UMP
-
?

Subunits

Subunits Comment Organism
? x * 26893, calculated from sequence Escherichia coli

Synonyms

Synonyms Comment Organism
LpxH
-
Escherichia coli
UDP-2,3-diacylglucosamine hydrolase
-
Escherichia coli
UDP-2,3-diacylglucosamine pyrophosphatase
-
Escherichia coli
YbbF
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 50% of maximal activity, pH 9.5: about 50% of maximal activity Escherichia coli