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show all sequences of 3.6.1.53

Characterization of Danio rerio Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase, the structural prototype of the ADPRibase-Mn-like protein family

Rodrigues, J.; Fernandez, A.; Canales, J.; Cabezas, A.; Ribeiro, J.; Costas, M.; Cameselle, J.; PLoS ONE 7, e42249 (2012)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
substrate-docking studies point to a role of His-97 in catalysis by orientation, and to a bidentate water bridging the dinuclear metal center as the potential nucleophile
Danio rerio
Engineering
Amino acid exchange
Commentary
Organism
H97A
severe reduction of activtiy
Danio rerio
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.071
-
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.19
-
cyclic ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.76
-
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
1.7
-
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
2
-
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
2.65
-
ADP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
3.9
-
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
6.3
-
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
12
-
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
19
-
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
31
-
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
43
-
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
about 25% of the activity with Mn2+
Danio rerio
Mn2+
required, similar activatory effects with all the substrates tested. Km values around 1 microM and 2.5 mM Mn2+ for ADP-ribose hydrolysis
Danio rerio
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Danio rerio
Q7T291
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',3'-cAMP + H2O
-
735057
Danio rerio
?
-
-
-
?
ADP + H2O
-
735057
Danio rerio
AMP + phosphate
-
-
-
?
ADP-ribose + H2O
-
735057
Danio rerio
AMP + D-ribose 5-phosphate
-
-
-
?
CDP-choline + H2O
-
735057
Danio rerio
CMP + phosphocholine
-
-
-
?
CDP-ethanolamine + H2O
-
735057
Danio rerio
CMP + phosphoethanolamine
-
-
-
?
CDP-glycerol + H2O
-
735057
Danio rerio
CMP + phosphoglycerol
-
-
-
?
cyclic ADP-ribose + H2O
-
735057
Danio rerio
N1-(5-phosphoribosyl)-AMP
-
-
-
?
additional information
very poor substrate: cyclic ADP-ribose. No substrate: ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, AMP or 3',5'-cAMP
735057
Danio rerio
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.005
-
cyclic ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.2
-
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.4
-
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.8
-
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.8
-
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
1.8
-
ADP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
2.1
-
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
16.9
-
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
18
-
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
29.7
-
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
67.2
-
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
95.6
-
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
125.2
-
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Danio rerio
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.6
-
half-maximum activity
Danio rerio
9.2
-
half-maximum activity
Danio rerio
Crystallization (Commentary) (protein specific)
Crystallization
Organism
substrate-docking studies point to a role of His-97 in catalysis by orientation, and to a bidentate water bridging the dinuclear metal center as the potential nucleophile
Danio rerio
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H97A
severe reduction of activtiy
Danio rerio
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.071
-
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.19
-
cyclic ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.76
-
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
1.7
-
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
2
-
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
2.65
-
ADP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
3.9
-
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
6.3
-
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
12
-
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
19
-
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
31
-
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
43
-
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
about 25% of the activity with Mn2+
Danio rerio
Mn2+
required, similar activatory effects with all the substrates tested. Km values around 1 microM and 2.5 mM Mn2+ for ADP-ribose hydrolysis
Danio rerio
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',3'-cAMP + H2O
-
735057
Danio rerio
?
-
-
-
?
ADP + H2O
-
735057
Danio rerio
AMP + phosphate
-
-
-
?
ADP-ribose + H2O
-
735057
Danio rerio
AMP + D-ribose 5-phosphate
-
-
-
?
CDP-choline + H2O
-
735057
Danio rerio
CMP + phosphocholine
-
-
-
?
CDP-ethanolamine + H2O
-
735057
Danio rerio
CMP + phosphoethanolamine
-
-
-
?
CDP-glycerol + H2O
-
735057
Danio rerio
CMP + phosphoglycerol
-
-
-
?
cyclic ADP-ribose + H2O
-
735057
Danio rerio
N1-(5-phosphoribosyl)-AMP
-
-
-
?
additional information
very poor substrate: cyclic ADP-ribose. No substrate: ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, AMP or 3',5'-cAMP
735057
Danio rerio
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.005
-
cyclic ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.2
-
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.4
-
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.8
-
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.8
-
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
1.8
-
ADP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
2.1
-
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
16.9
-
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
18
-
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
29.7
-
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
67.2
-
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
95.6
-
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
125.2
-
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Danio rerio
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.6
-
half-maximum activity
Danio rerio
9.2
-
half-maximum activity
Danio rerio
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.011
-
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.02
-
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.028
-
cyclic ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.07
-
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.12
-
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.66
-
ADP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
5.6
-
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
5.9
-
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
18
-
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
22
-
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
32
-
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
48
-
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
340
-
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.011
-
ADP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.02
-
CDP-choline
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.028
-
cyclic ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
0.07
-
CDP-ethanolamine
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.12
-
CDP-glycerol
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
0.66
-
ADP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
5.6
-
CDP-choline
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
5.9
-
ADP-ribose
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
18
-
2',3'-cAMP
mutant H97A, pH 7.5, temperature not specified in the publication
Danio rerio
22
-
2',3'-cAMP
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
32
-
CDP-ethanolamine
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
48
-
CDP-glycerol
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
340
-
ADP-ribose
wild-type, pH 7.5, temperature not specified in the publication
Danio rerio
Other publictions for EC 3.6.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735009
Cabezas
Molecular bases of catalysis a ...
Homo sapiens
PLoS ONE
10
e0118680
2015
-
-
-
1
13
-
-
42
-
-
-
-
-
7
-
-
-
-
-
1
-
-
4
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
1
13
-
-
-
-
42
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
44
-
-
-
-
-
-
-
-
54
54
735057
Rodrigues
Characterization of Danio reri ...
Danio rerio
PLoS ONE
7
e42249
2012
-
-
-
1
1
-
-
13
-
2
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
13
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
13
-
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
13
1
2
-
-
-
-
-
-
13
13
697946
Canales
Hydrolysis of the phosphoanhyd ...
Rattus norvegicus
FEBS Lett.
583
1593-1598
2009
-
-
-
1
-
-
-
1
-
1
-
3
-
3
-
-
-
-
-
-
1
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
1
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
690823
Canales
Mn2+-dependent ADP-ribose/CDP- ...
Mus musculus, Rattus norvegicus
Biochem. J.
413
103-113
2008
-
-
1
1
-
-
2
5
-
2
4
-
-
6
-
-
1
-
-
7
-
1
11
1
-
-
-
5
2
1
1
-
-
-
-
-
-
1
-
1
-
-
-
2
-
5
-
2
4
-
-
-
-
1
-
7
-
1
11
1
-
-
-
5
2
1
1
-
-
-
-
-
-
-
209868
Canales
Rat liver nucleoside diphospho ...
Rattus norvegicus
Biochim. Biophys. Acta
1246
167-177
1995
-
-
-
-
-
-
-
1
-
2
1
-
-
1
-
-
1
-
-
1
1
-
10
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
1
-
1
1
-
10
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-