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Literature summary for 3.6.1.53 extracted from

  • Rodrigues, J.; Fernandez, A.; Canales, J.; Cabezas, A.; Ribeiro, J.; Costas, M.; Cameselle, J.
    Characterization of Danio rerio Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase, the structural prototype of the ADPRibase-Mn-like protein family (2012), PLoS ONE, 7, e42249.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.53

Crystallization (Commentary)

Crystallization (Comment) Organism
substrate-docking studies point to a role of His-97 in catalysis by orientation, and to a bidentate water bridging the dinuclear metal center as the potential nucleophile Danio rerio

Protein Variants

Protein Variants Comment Organism
H97A severe reduction of activtiy Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 ADP-ribose wild-type, pH 7.5, temperature not specified in the publication Danio rerio
0.071
-
 ADP-ribose mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.19
-
 cyclic ADP-ribose wild-type, pH 7.5, temperature not specified in the publication Danio rerio
0.76
-
 2',3'-cAMP wild-type, pH 7.5, temperature not specified in the publication Danio rerio
1.7
-
 2',3'-cAMP mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
2
-
 CDP-glycerol wild-type, pH 7.5, temperature not specified in the publication Danio rerio
2.65
-
 ADP wild-type, pH 7.5, temperature not specified in the publication Danio rerio
3.9
-
 CDP-ethanolamine wild-type, pH 7.5, temperature not specified in the publication Danio rerio
6.3
-
 CDP-glycerol mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
12
-
 CDP-choline wild-type, pH 7.5, temperature not specified in the publication Danio rerio
19
-
 ADP mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
31
-
 CDP-ethanolamine mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
43
-
 CDP-choline mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 25% of the activity with Mn2+ Danio rerio
Mn2+ required, similar activatory effects with all the substrates tested. Km values around 1 microM and 2.5 mM Mn2+ for ADP-ribose hydrolysis Danio rerio

Organism

Organism UniProt Comment Textmining
Danio rerio Q7T291
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cAMP + H2O
-
 Danio rerio ?
-
 ?
ADP + H2O
-
 Danio rerio AMP + phosphate
-
 ?
ADP-ribose + H2O
-
 Danio rerio AMP + D-ribose 5-phosphate
-
 ?
CDP-choline + H2O
-
 Danio rerio CMP + phosphocholine
-
 ?
CDP-ethanolamine + H2O
-
 Danio rerio CMP + phosphoethanolamine
-
 ?
CDP-glycerol + H2O
-
 Danio rerio CMP + phosphoglycerol
-
 ?
cyclic ADP-ribose + H2O
-
 Danio rerio N1-(5-phosphoribosyl)-AMP
-
 ?
additional information very poor substrate: cyclic ADP-ribose. No substrate: ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, AMP or 3',5'-cAMP Danio rerio ?
-
 ?

Synonyms

Synonyms Comment Organism
ADPRibase-Mn
-
 Danio rerio
Adprm
-
 Danio rerio

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 cyclic ADP-ribose wild-type, pH 7.5, temperature not specified in the publication Danio rerio
0.2
-
 ADP mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.4
-
 ADP-ribose mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.8
-
 CDP-choline mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.8
-
 CDP-glycerol mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
1.8
-
 ADP wild-type, pH 7.5, temperature not specified in the publication Danio rerio
2.1
-
 CDP-ethanolamine mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
16.9
-
 2',3'-cAMP wild-type, pH 7.5, temperature not specified in the publication Danio rerio
18
-
 ADP-ribose wild-type, pH 7.5, temperature not specified in the publication Danio rerio
29.7
-
 2',3'-cAMP mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
67.2
-
 CDP-choline wild-type, pH 7.5, temperature not specified in the publication Danio rerio
95.6
-
 CDP-glycerol wild-type, pH 7.5, temperature not specified in the publication Danio rerio
125.2
-
 CDP-ethanolamine wild-type, pH 7.5, temperature not specified in the publication Danio rerio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Danio rerio

pH Range

pH Minimum pH Maximum Comment Organism
7.6
-
 half-maximum activity Danio rerio
9.2
-
 half-maximum activity Danio rerio

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.011
-
 ADP mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.02
-
 CDP-choline mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.028
-
 cyclic ADP-ribose wild-type, pH 7.5, temperature not specified in the publication Danio rerio
0.07
-
 CDP-ethanolamine mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.12
-
 CDP-glycerol mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
0.66
-
 ADP wild-type, pH 7.5, temperature not specified in the publication Danio rerio
5.6
-
 CDP-choline wild-type, pH 7.5, temperature not specified in the publication Danio rerio
5.9
-
 ADP-ribose mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
18
-
 2',3'-cAMP mutant H97A, pH 7.5, temperature not specified in the publication Danio rerio
22
-
 2',3'-cAMP wild-type, pH 7.5, temperature not specified in the publication Danio rerio
32
-
 CDP-ethanolamine wild-type, pH 7.5, temperature not specified in the publication Danio rerio
48
-
 CDP-glycerol wild-type, pH 7.5, temperature not specified in the publication Danio rerio
340
-
 ADP-ribose wild-type, pH 7.5, temperature not specified in the publication Danio rerio