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show all sequences of 3.6.1.53

Molecular bases of catalysis and ADP-ribose preference of human Mn2+-dependent adpribose/CDP-alcohol diphosphatase and conversion by mutagenesis to a preferential cyclic ADP-ribose phosphohydrolase

Cabezas, A.; Ribeiro, J.; Rodrigues, J.; Lopez-Villamizar, I.; Fernandez, A.; Canales, J.; Pinto, R.; Costas, M.; Cameselle, J.; PLoS ONE 10, e0118680 (2015)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
substrate docking on a homology model of human ADPRibase-Mn suggests possible interactions of ADP-ribose with residue Cys253, within the metallo-dependent phosphatases signature with Gln27, Asn110, His111, or in unique structural regions of the ADPRibase-Mn family, residues Phe37 and Arg43 and Phe210
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C253A
10fold increase of the catalytic efficiency for cyclic ADP-ribose; Cys253 is hindering for cADPR phosphohydrolase activity, specific tenfold gain of efficiency with cyclic ADP-ribose
Homo sapiens
F210A
mutation lowers 40-70fold the catalytic efficiency for ADP-ribose, CDP-choline and 2',3'-cAMP hydrolysis, and 500fold for cyclic ADP-ribose
Homo sapiens
F37A
Phe37 is needed for ADP-ribose preference without catalytic effect
Homo sapiens
F37A/L196A
modest enhancement of the inhibitory effect over that of the F37A point mutation
Homo sapiens
F37A/L196F
modest enhancement of the inhibitory effect over that of the F37A point mutation
Homo sapiens
F37A/L196F/C253A
cyclic ADP-ribose is the best substrate, with a 8fold increase in catalytic efficiency compared to wild-type
Homo sapiens
F37Y
kinetic parameters similar to wild-type
Homo sapiens
H111A
marked decrease in efficiency with all substrates except 2',3'-cAMP
Homo sapiens
H111N
marked decrease in efficiency with all substrates except 2',3'-cAMP
Homo sapiens
L196A
modest 2-5fold decrease of catalytic efficiency with the substrates tested
Homo sapiens
N110A
reduction of catalytic efficiency is stronger for the hydrolysis of CDP-choline or 2',3'-cAMP than for the hydrolysis of ADP-ribose or cADPR. The decrease of kcat value is stronger for the hydrolysis of 2',3'-cAMP
Homo sapiens
Q27H
for ADP-ribose, modest negative effects of similar magnitude in catalysis and in substrate binding. For CDP-choline, the substitution affects only binding. For 2',3'-cAMP, the substitution affects catalysis, not binding
Homo sapiens
R43A
Arg43 is essential for catalysis, drastic efficiency loss of the mutant
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
0.077
-
ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
0.078
-
ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
0.094
-
ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
0.115
-
ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.13
-
ADP-ribose
mutant L196A, pH 7.5, 37C
Homo sapiens
0.14
-
ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
0.2
-
ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
0.2
-
cyclic ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
0.29
-
ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
0.35
-
CDP-choline
wild-type, pH 7.5, 37C
Homo sapiens
0.43
-
CDP-choline
mutant F37Y, pH 7.5, 37C
Homo sapiens
0.46
-
cyclic ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
0.47
-
CDP-choline
mutant L196A, pH 7.5, 37C
Homo sapiens
0.5
-
CDP-choline
mutant C253A, pH 7.5, 37C
Homo sapiens
0.78
-
cyclic ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
0.97
-
CDP-choline
mutant F37A, pH 7.5, 37C
Homo sapiens
1.05
-
2',3'-cAMP
mutant N110A, pH 7.5, 37C
Homo sapiens
1.15
-
ADP-ribose
mutant F37A/L196A, pH 7.5, 37C; mutant F37A, pH 7.5, 37C
Homo sapiens
1.2
-
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
1.25
-
2',3'-cAMP
mutant H111A, pH 7.5, 37C
Homo sapiens
1.25
-
CDP-choline
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
1.5
-
CDP-choline
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
1.6
-
ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
1.7
-
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
1.8
-
2',3'-cAMP
mutant H111N, pH 7.5, 37C
Homo sapiens
2.1
-
ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
2.3
-
2',3'-cAMP
mutant Q27H, pH 7.5, 37C
Homo sapiens
2.4
-
2',3'-cAMP
wild-type, pH 7.5, 37C
Homo sapiens
2.5
-
2',3'-cAMP
mutant F37Y, pH 7.5, 37C
Homo sapiens
2.6
-
2',3'-cAMP
mutant C253A, pH 7.5, 37C
Homo sapiens
2.7
-
CDP-choline
mutant H111N, pH 7.5, 37C; mutant Q27H, pH 7.5, 37C
Homo sapiens
3.4
-
2',3'-cAMP
mutant L196A, pH 7.5, 37C
Homo sapiens
4
-
CDP-choline
mutant N110A, pH 7.5, 37C
Homo sapiens
4.8
-
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
5.1
-
2',3'-cAMP
mutant F37A, pH 7.5, 37C
Homo sapiens
5.2
-
2',3'-cAMP
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
7.1
-
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
7.5
-
CDP-choline
mutant H111A, pH 7.5, 37C
Homo sapiens
7.6
-
2',3'-cAMP
mutant F210A, pH 7.5, 37C; mutant R43A, pH 7.5, 37C
Homo sapiens
9
-
CDP-choline
mutant R43A, pH 7.5, 37C
Homo sapiens
11
-
CDP-choline
mutant F210A, pH 7.5, 37C
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
W0NWJ0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',3'-cAMP + H2O
-
735009
Homo sapiens
?
-
-
-
?
ADP-ribose + H2O
-
735009
Homo sapiens
AMP + D-ribose 5-phosphate
-
-
-
?
CDP-choline + H2O
-
735009
Homo sapiens
CMP + phosphocholine
-
-
-
?
cyclic ADP-ribose + H2O
-
735009
Homo sapiens
N1-(5-phosphoribosyl)-AMP
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.003
-
ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.025
-
CDP-choline
mutant R43A, pH 7.5, 37C
Homo sapiens
0.11
-
2',3'-cAMP
mutant N110A, pH 7.5, 37C
Homo sapiens
0.47
-
ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
0.95
-
2',3'-cAMP
mutant R43A, pH 7.5, 37C
Homo sapiens
2
8
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
2.2
-
CDP-choline
mutant H111A, pH 7.5, 37C
Homo sapiens
2.3
-
ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
2.7
-
2',3'-cAMP
mutant F210A, pH 7.5, 37C
Homo sapiens
3
6
2',3'-cAMP
mutant F37A, pH 7.5, 37C
Homo sapiens
3.2
-
cyclic ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
4.3
-
2',3'-cAMP
mutant Q27H, pH 7.5, 37C
Homo sapiens
4.3
-
ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
5.3
-
CDP-choline
mutant H111N, pH 7.5, 37C
Homo sapiens
5.7
-
CDP-choline
mutant N110A, pH 7.5, 37C
Homo sapiens
7
-
ADP-ribose
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
7.4
-
2',3'-cAMP
mutant H111A, pH 7.5, 37C
Homo sapiens
8
-
ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
8.9
-
cyclic ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
10
-
ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
13
-
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
14
-
ADP-ribose
mutant F37A, pH 7.5, 37C
Homo sapiens
16
-
ADP-ribose
mutant L196A, pH 7.5, 37C
Homo sapiens
16
-
cyclic ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
19
-
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
19
-
ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
20
-
2',3'-cAMP
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
25
-
CDP-choline
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
26
-
CDP-choline
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
29
-
CDP-choline
mutant L196A, pH 7.5, 37C
Homo sapiens
30
-
2',3'-cAMP
mutant L196A, pH 7.5, 37C
Homo sapiens
33
-
CDP-choline
mutant F37A, pH 7.5, 37C
Homo sapiens
34
-
2',3'-cAMP
mutant H111N, pH 7.5, 37C
Homo sapiens
35
-
ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
37
-
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
39
-
CDP-choline
mutant Q27H, pH 7.5, 37C
Homo sapiens
50
-
ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
50
-
CDP-choline
mutant F210A, pH 7.5, 37C; wild-type, pH 7.5, 37C
Homo sapiens
60
-
2',3'-cAMP
wild-type, pH 7.5, 37C
Homo sapiens
70
-
2',3'-cAMP
mutant F37Y, pH 7.5, 37C
Homo sapiens
76
-
CDP-choline
mutant F37Y, pH 7.5, 37C
Homo sapiens
79
-
CDP-choline
mutant C253A, pH 7.5, 37C
Homo sapiens
83
-
2',3'-cAMP
mutant C253A, pH 7.5, 37C
Homo sapiens
97
-
ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
substrate docking on a homology model of human ADPRibase-Mn suggests possible interactions of ADP-ribose with residue Cys253, within the metallo-dependent phosphatases signature with Gln27, Asn110, His111, or in unique structural regions of the ADPRibase-Mn family, residues Phe37 and Arg43 and Phe210
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C253A
10fold increase of the catalytic efficiency for cyclic ADP-ribose; Cys253 is hindering for cADPR phosphohydrolase activity, specific tenfold gain of efficiency with cyclic ADP-ribose
Homo sapiens
F210A
mutation lowers 40-70fold the catalytic efficiency for ADP-ribose, CDP-choline and 2',3'-cAMP hydrolysis, and 500fold for cyclic ADP-ribose
Homo sapiens
F37A
Phe37 is needed for ADP-ribose preference without catalytic effect
Homo sapiens
F37A/L196A
modest enhancement of the inhibitory effect over that of the F37A point mutation
Homo sapiens
F37A/L196F
modest enhancement of the inhibitory effect over that of the F37A point mutation
Homo sapiens
F37A/L196F/C253A
cyclic ADP-ribose is the best substrate, with a 8fold increase in catalytic efficiency compared to wild-type
Homo sapiens
F37Y
kinetic parameters similar to wild-type
Homo sapiens
H111A
marked decrease in efficiency with all substrates except 2',3'-cAMP
Homo sapiens
H111N
marked decrease in efficiency with all substrates except 2',3'-cAMP
Homo sapiens
L196A
modest 2-5fold decrease of catalytic efficiency with the substrates tested
Homo sapiens
N110A
reduction of catalytic efficiency is stronger for the hydrolysis of CDP-choline or 2',3'-cAMP than for the hydrolysis of ADP-ribose or cADPR. The decrease of kcat value is stronger for the hydrolysis of 2',3'-cAMP
Homo sapiens
Q27H
for ADP-ribose, modest negative effects of similar magnitude in catalysis and in substrate binding. For CDP-choline, the substitution affects only binding. For 2',3'-cAMP, the substitution affects catalysis, not binding
Homo sapiens
R43A
Arg43 is essential for catalysis, drastic efficiency loss of the mutant
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
0.077
-
ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
0.078
-
ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
0.094
-
ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
0.115
-
ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.13
-
ADP-ribose
mutant L196A, pH 7.5, 37C
Homo sapiens
0.14
-
ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
0.2
-
ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
0.2
-
cyclic ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
0.29
-
ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
0.35
-
CDP-choline
wild-type, pH 7.5, 37C
Homo sapiens
0.43
-
CDP-choline
mutant F37Y, pH 7.5, 37C
Homo sapiens
0.46
-
cyclic ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
0.47
-
CDP-choline
mutant L196A, pH 7.5, 37C
Homo sapiens
0.5
-
CDP-choline
mutant C253A, pH 7.5, 37C
Homo sapiens
0.78
-
cyclic ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
0.97
-
CDP-choline
mutant F37A, pH 7.5, 37C
Homo sapiens
1.05
-
2',3'-cAMP
mutant N110A, pH 7.5, 37C
Homo sapiens
1.15
-
ADP-ribose
mutant F37A/L196A, pH 7.5, 37C; mutant F37A, pH 7.5, 37C
Homo sapiens
1.2
-
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
1.25
-
2',3'-cAMP
mutant H111A, pH 7.5, 37C
Homo sapiens
1.25
-
CDP-choline
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
1.5
-
CDP-choline
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
1.6
-
ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
1.7
-
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
1.8
-
2',3'-cAMP
mutant H111N, pH 7.5, 37C
Homo sapiens
2.1
-
ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
2.3
-
2',3'-cAMP
mutant Q27H, pH 7.5, 37C
Homo sapiens
2.4
-
2',3'-cAMP
wild-type, pH 7.5, 37C
Homo sapiens
2.5
-
2',3'-cAMP
mutant F37Y, pH 7.5, 37C
Homo sapiens
2.6
-
2',3'-cAMP
mutant C253A, pH 7.5, 37C
Homo sapiens
2.7
-
CDP-choline
mutant H111N, pH 7.5, 37C; mutant Q27H, pH 7.5, 37C
Homo sapiens
3.4
-
2',3'-cAMP
mutant L196A, pH 7.5, 37C
Homo sapiens
4
-
CDP-choline
mutant N110A, pH 7.5, 37C
Homo sapiens
4.8
-
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
5.1
-
2',3'-cAMP
mutant F37A, pH 7.5, 37C
Homo sapiens
5.2
-
2',3'-cAMP
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
7.1
-
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
7.5
-
CDP-choline
mutant H111A, pH 7.5, 37C
Homo sapiens
7.6
-
2',3'-cAMP
mutant F210A, pH 7.5, 37C; mutant R43A, pH 7.5, 37C
Homo sapiens
9
-
CDP-choline
mutant R43A, pH 7.5, 37C
Homo sapiens
11
-
CDP-choline
mutant F210A, pH 7.5, 37C
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',3'-cAMP + H2O
-
735009
Homo sapiens
?
-
-
-
?
ADP-ribose + H2O
-
735009
Homo sapiens
AMP + D-ribose 5-phosphate
-
-
-
?
CDP-choline + H2O
-
735009
Homo sapiens
CMP + phosphocholine
-
-
-
?
cyclic ADP-ribose + H2O
-
735009
Homo sapiens
N1-(5-phosphoribosyl)-AMP
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.003
-
ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.025
-
CDP-choline
mutant R43A, pH 7.5, 37C
Homo sapiens
0.11
-
2',3'-cAMP
mutant N110A, pH 7.5, 37C
Homo sapiens
0.47
-
ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
0.95
-
2',3'-cAMP
mutant R43A, pH 7.5, 37C
Homo sapiens
2
8
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
2.2
-
CDP-choline
mutant H111A, pH 7.5, 37C
Homo sapiens
2.3
-
ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
2.7
-
2',3'-cAMP
mutant F210A, pH 7.5, 37C
Homo sapiens
3
6
2',3'-cAMP
mutant F37A, pH 7.5, 37C
Homo sapiens
3.2
-
cyclic ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
4.3
-
2',3'-cAMP
mutant Q27H, pH 7.5, 37C
Homo sapiens
4.3
-
ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
5.3
-
CDP-choline
mutant H111N, pH 7.5, 37C
Homo sapiens
5.7
-
CDP-choline
mutant N110A, pH 7.5, 37C
Homo sapiens
7
-
ADP-ribose
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
7.4
-
2',3'-cAMP
mutant H111A, pH 7.5, 37C
Homo sapiens
8
-
ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
8.9
-
cyclic ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
10
-
ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
13
-
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
14
-
ADP-ribose
mutant F37A, pH 7.5, 37C
Homo sapiens
16
-
ADP-ribose
mutant L196A, pH 7.5, 37C
Homo sapiens
16
-
cyclic ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
19
-
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
19
-
ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
20
-
2',3'-cAMP
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
25
-
CDP-choline
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
26
-
CDP-choline
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
29
-
CDP-choline
mutant L196A, pH 7.5, 37C
Homo sapiens
30
-
2',3'-cAMP
mutant L196A, pH 7.5, 37C
Homo sapiens
33
-
CDP-choline
mutant F37A, pH 7.5, 37C
Homo sapiens
34
-
2',3'-cAMP
mutant H111N, pH 7.5, 37C
Homo sapiens
35
-
ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
37
-
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
39
-
CDP-choline
mutant Q27H, pH 7.5, 37C
Homo sapiens
50
-
ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
50
-
CDP-choline
mutant F210A, pH 7.5, 37C; wild-type, pH 7.5, 37C
Homo sapiens
60
-
2',3'-cAMP
wild-type, pH 7.5, 37C
Homo sapiens
70
-
2',3'-cAMP
mutant F37Y, pH 7.5, 37C
Homo sapiens
76
-
CDP-choline
mutant F37Y, pH 7.5, 37C
Homo sapiens
79
-
CDP-choline
mutant C253A, pH 7.5, 37C
Homo sapiens
83
-
2',3'-cAMP
mutant C253A, pH 7.5, 37C
Homo sapiens
97
-
ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0001
-
cyclic ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.006
-
CDP-choline
mutant R43A, pH 7.5, 37C
Homo sapiens
0.03
-
ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.07
-
cyclic ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
0.08
-
cyclic ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
0.1
-
2',3'-cAMP
mutant N110A, pH 7.5, 37C
Homo sapiens
0.13
-
2',3'-cAMP
mutant R43A, pH 7.5, 37C
Homo sapiens
0.13
-
cyclic ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
0.15
-
cyclic ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
0.29
-
cyclic ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
0.3
-
CDP-choline
mutant H111A, pH 7.5, 37C
Homo sapiens
0.4
-
2',3'-cAMP
mutant F210A, pH 7.5, 37C
Homo sapiens
0.9
-
cyclic ADP-ribose
mutant F37A/L196A, pH 7.5, 37C; mutant L196A, pH 7.5, 37C
Homo sapiens
1.3
-
cyclic ADP-ribose
mutant F37A, pH 7.5, 37C
Homo sapiens
1.5
-
CDP-choline
mutant N110A, pH 7.5, 37C
Homo sapiens
1.6
-
cyclic ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
1.9
-
2',3'-cAMP
mutant Q27H, pH 7.5, 37C
Homo sapiens
2
-
CDP-choline
mutant H111N, pH 7.5, 37C
Homo sapiens
3
-
ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
3.9
-
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
4
-
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37C; mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
4
-
CDP-choline
mutant F210A, pH 7.5, 37C
Homo sapiens
4
-
cyclic ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
4.1
-
cyclic ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
4.8
-
ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
6
-
2',3'-cAMP
mutant H111A, pH 7.5, 37C
Homo sapiens
6
-
ADP-ribose
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
7
-
2',3'-cAMP
mutant F37A, pH 7.5, 37C
Homo sapiens
9
-
2',3'-cAMP
mutant L196A, pH 7.5, 37C
Homo sapiens
9
-
ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
11
-
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
12
-
ADP-ribose
mutant F37A, pH 7.5, 37C
Homo sapiens
14
-
CDP-choline
mutant Q27H, pH 7.5, 37C
Homo sapiens
15
-
ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
17
-
CDP-choline
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
19
-
2',3'-cAMP
mutant H111N, pH 7.5, 37C
Homo sapiens
21
-
CDP-choline
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
22
-
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
25
-
2',3'-cAMP
wild-type, pH 7.5, 37C
Homo sapiens
28
-
2',3'-cAMP
mutant F37Y, pH 7.5, 37C
Homo sapiens
29
-
ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
32
-
2',3'-cAMP
mutant C253A, pH 7.5, 37C
Homo sapiens
34
-
CDP-choline
mutant F37A, pH 7.5, 37C
Homo sapiens
35.5
-
cyclic ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
44
-
cyclic ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
52
-
ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
62
-
CDP-choline
mutant L196A, pH 7.5, 37C
Homo sapiens
120
-
ADP-ribose
mutant L196A, pH 7.5, 37C
Homo sapiens
150
-
CDP-choline
wild-type, pH 7.5, 37C
Homo sapiens
160
-
CDP-choline
mutant C253A, pH 7.5, 37C
Homo sapiens
180
-
CDP-choline
mutant F37Y, pH 7.5, 37C
Homo sapiens
590
-
ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
650
-
ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
1000
-
ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0001
-
cyclic ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.006
-
CDP-choline
mutant R43A, pH 7.5, 37C
Homo sapiens
0.03
-
ADP-ribose
mutant R43A, pH 7.5, 37C
Homo sapiens
0.07
-
cyclic ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
0.08
-
cyclic ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
0.1
-
2',3'-cAMP
mutant N110A, pH 7.5, 37C
Homo sapiens
0.13
-
2',3'-cAMP
mutant R43A, pH 7.5, 37C
Homo sapiens
0.13
-
cyclic ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
0.15
-
cyclic ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
0.29
-
cyclic ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
0.3
-
CDP-choline
mutant H111A, pH 7.5, 37C
Homo sapiens
0.4
-
2',3'-cAMP
mutant F210A, pH 7.5, 37C
Homo sapiens
0.9
-
cyclic ADP-ribose
mutant F37A/L196A, pH 7.5, 37C; mutant L196A, pH 7.5, 37C
Homo sapiens
1.3
-
cyclic ADP-ribose
mutant F37A, pH 7.5, 37C
Homo sapiens
1.5
-
CDP-choline
mutant N110A, pH 7.5, 37C
Homo sapiens
1.6
-
cyclic ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
1.9
-
2',3'-cAMP
mutant Q27H, pH 7.5, 37C
Homo sapiens
2
-
CDP-choline
mutant H111N, pH 7.5, 37C
Homo sapiens
3
-
ADP-ribose
mutant H111A, pH 7.5, 37C
Homo sapiens
3.9
-
2',3'-cAMP
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
4
-
2',3'-cAMP
mutant F37A/L196A, pH 7.5, 37C; mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
4
-
CDP-choline
mutant F210A, pH 7.5, 37C
Homo sapiens
4
-
cyclic ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
4.1
-
cyclic ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
4.8
-
ADP-ribose
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
6
-
2',3'-cAMP
mutant H111A, pH 7.5, 37C
Homo sapiens
6
-
ADP-ribose
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
7
-
2',3'-cAMP
mutant F37A, pH 7.5, 37C
Homo sapiens
9
-
2',3'-cAMP
mutant L196A, pH 7.5, 37C
Homo sapiens
9
-
ADP-ribose
mutant F210A, pH 7.5, 37C
Homo sapiens
11
-
ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
12
-
ADP-ribose
mutant F37A, pH 7.5, 37C
Homo sapiens
14
-
CDP-choline
mutant Q27H, pH 7.5, 37C
Homo sapiens
15
-
ADP-ribose
mutant N110A, pH 7.5, 37C
Homo sapiens
17
-
CDP-choline
mutant F37A/L196F, pH 7.5, 37C
Homo sapiens
19
-
2',3'-cAMP
mutant H111N, pH 7.5, 37C
Homo sapiens
21
-
CDP-choline
mutant F37A/L196A, pH 7.5, 37C
Homo sapiens
22
-
CDP-choline
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
25
-
2',3'-cAMP
wild-type, pH 7.5, 37C
Homo sapiens
28
-
2',3'-cAMP
mutant F37Y, pH 7.5, 37C
Homo sapiens
29
-
ADP-ribose
mutant H111N, pH 7.5, 37C
Homo sapiens
32
-
2',3'-cAMP
mutant C253A, pH 7.5, 37C
Homo sapiens
34
-
CDP-choline
mutant F37A, pH 7.5, 37C
Homo sapiens
35.5
-
cyclic ADP-ribose
mutant F37A/L196F/C253A, pH 7.5, 37C
Homo sapiens
44
-
cyclic ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
52
-
ADP-ribose
mutant Q27H, pH 7.5, 37C
Homo sapiens
62
-
CDP-choline
mutant L196A, pH 7.5, 37C
Homo sapiens
120
-
ADP-ribose
mutant L196A, pH 7.5, 37C
Homo sapiens
150
-
CDP-choline
wild-type, pH 7.5, 37C
Homo sapiens
160
-
CDP-choline
mutant C253A, pH 7.5, 37C
Homo sapiens
180
-
CDP-choline
mutant F37Y, pH 7.5, 37C
Homo sapiens
590
-
ADP-ribose
wild-type, pH 7.5, 37C
Homo sapiens
650
-
ADP-ribose
mutant F37Y, pH 7.5, 37C
Homo sapiens
1000
-
ADP-ribose
mutant C253A, pH 7.5, 37C
Homo sapiens
Other publictions for EC 3.6.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735009
Cabezas
Molecular bases of catalysis a ...
Homo sapiens
PLoS ONE
10
e0118680
2015
-
-
-
1
13
-
-
42
-
-
-
-
-
7
-
-
-
-
-
1
-
-
4
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
1
13
-
-
-
-
42
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
44
-
-
-
-
-
-
-
-
54
54
735057
Rodrigues
Characterization of Danio reri ...
Danio rerio
PLoS ONE
7
e42249
2012
-
-
-
1
1
-
-
13
-
2
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
13
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
13
-
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
13
1
2
-
-
-
-
-
-
13
13
697946
Canales
Hydrolysis of the phosphoanhyd ...
Rattus norvegicus
FEBS Lett.
583
1593-1598
2009
-
-
-
1
-
-
-
1
-
1
-
3
-
3
-
-
-
-
-
-
1
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
1
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
690823
Canales
Mn2+-dependent ADP-ribose/CDP- ...
Mus musculus, Rattus norvegicus
Biochem. J.
413
103-113
2008
-
-
1
1
-
-
2
5
-
2
4
-
-
6
-
-
1
-
-
7
-
1
11
1
-
-
-
5
2
1
1
-
-
-
-
-
-
1
-
1
-
-
-
2
-
5
-
2
4
-
-
-
-
1
-
7
-
1
11
1
-
-
-
5
2
1
1
-
-
-
-
-
-
-
209868
Canales
Rat liver nucleoside diphospho ...
Rattus norvegicus
Biochim. Biophys. Acta
1246
167-177
1995
-
-
-
-
-
-
-
1
-
2
1
-
-
1
-
-
1
-
-
1
1
-
10
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
1
-
1
1
-
10
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-