BRENDA - Enzyme Database show
show all sequences of 3.6.1.53

Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase: a novel metallophosphoesterase family preferentially expressed in rodent immune cells

Canales, J.; Fernandez, A.; Ribeiro, J.M.; Cabezas, A.; Rodrigues, J.R.; Cameselle, J.C.; Costas, M.J.; Biochem. J. 413, 103-113 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 cells; expression as GST-fusion protein
Rattus norvegicus
Crystallization (Commentary)
Crystallization
Organism
homology modeling based on crystal structure of the Danio rerio protein, Protein Data Base entry 2NXF, and docking of ADP-ribose
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
incubation with 2.5 mM EDTA does not affect ADPRibase-Mn activity; not inhibitory: EDTA up to 2.5 mM
Rattus norvegicus
o-phenanthroline
5 mM causes near full inactivation in 1.5 h
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.039
-
ADP-ribose
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
0.201
-
ADP
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
0.3
-
CDP-choline
pH 7.5, 37°C; recombinant, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
0.304
-
CDP-glycerol
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
1.422
-
CDP-ethanolamine
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
dependent on, ADPRibase-Mn is inactive without added Mn2+, and it shows a high affinity for the cation, since saturation is observed at low-micromolar concentrations of Mn2+ well below the substrate concentration (0.5 mM), in no case can Mg2+ replace Mn2+; required. Concentration of MnCl2 giving the half-maximal rate is 0.001-0.004 mM. Mn2+ cannot be replaced by Mg2+
Rattus norvegicus
additional information
Mg2+ cannot replace Mn2+
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Rattus norvegicus
38000
-
SDS-PAGE
Rattus norvegicus
39360
-
calculated from amino acid sequence
Rattus norvegicus
40000
-
1 * 39360, calculated, 1 * 40000, SDS-PAGE, enzyme plus N-terminal extension GPLGSPNS left after proteolysis of recombinant fusion protein
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q99KS6
-
-
Rattus norvegicus
Q5M886
; bifunctional Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase
-
Purification (Commentary)
Commentary
Organism
glutathione-Sepharose column chromatography; recombinant GSt-fusion protein, in-column proteolysis of fusion
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
additional information
preferential expression in immune cells
Mus musculus
-
skeletal muscle
-
Rattus norvegicus
-
spleen
ADPRibase-Mn activity in spleen is 2.5-5fold higher than in liver and muscle
Rattus norvegicus
-
splenocyte
-
Rattus norvegicus
-
thymus
ADPRibase-Mn activity in thymus is 2.5-5fold higher than in liver and muscle
Rattus norvegicus
-
Storage Stability
Storage Stability
Organism
-20°C, 1 mM EDTA, stable for months
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
27% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
?
-
-
-
?
ADP + H2O
26% of the activity with ADP-ribose
690823
Rattus norvegicus
AMP + phosphate
-
-
-
?
ADP-ribose + H2O
-
690823
Rattus norvegicus
AMP + D-ribose 5-phosphate
-
-
-
?
ADP-ribose + H2O
100% activity at 0.5 mM substrate
690823
Rattus norvegicus
AMP + D-ribose 5-phosphate
-
-
-
?
CDP-choline + H2O
156% of the activity with ADP-ribose
690823
Rattus norvegicus
CMP + phosphocholine
-
-
-
?
CDP-choline + H2O
140% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
CMP + phosphocholine
-
-
-
?
CDP-ethanolamine + H2O
84% of the activity with ADP-ribose
690823
Rattus norvegicus
CMP + phosphoethanolamine
-
-
-
?
CDP-ethanolamine + H2O
89% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
?
-
-
-
?
CDP-glycerol + H2O
103% of the activity with ADP-ribose
690823
Rattus norvegicus
CMP + phosphoglycerol
-
-
-
?
CDP-glycerol + H2O
89% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
?
-
-
-
?
additional information
ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, and AMP are not hydrolysed
690823
Rattus norvegicus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 30000, gel filtration; 1 * 39360, calculated, 1 * 40000, SDS-PAGE, enzyme plus N-terminal extension GPLGSPNS left after proteolysis of recombinant fusion protein
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4
-
ADP
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
12.8
-
ADP-ribose
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 01 mM MnCl2, at 37°C
Rattus norvegicus
28.1
-
CDP-glycerol
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
28.5
-
CDP-choline
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
41.3
-
CDP-ethanolamine
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8
activity towards ADP- and CDP-alcohols
Rattus norvegicus
8
8.5
; ADP-ribose hydrolase activity
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
-
Rattus norvegicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
the activities towards ADP and CDP-alcohols are highest at pH 6-8 and decrease abruptly at higher pH
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 cells; expression as GST-fusion protein
Rattus norvegicus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
homology modeling based on crystal structure of the Danio rerio protein, Protein Data Base entry 2NXF, and docking of ADP-ribose
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
incubation with 2.5 mM EDTA does not affect ADPRibase-Mn activity; not inhibitory: EDTA up to 2.5 mM
Rattus norvegicus
o-phenanthroline
5 mM causes near full inactivation in 1.5 h
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.039
-
ADP-ribose
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
0.201
-
ADP
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
0.3
-
CDP-choline
pH 7.5, 37°C; recombinant, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
0.304
-
CDP-glycerol
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
1.422
-
CDP-ethanolamine
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
dependent on, ADPRibase-Mn is inactive without added Mn2+, and it shows a high affinity for the cation, since saturation is observed at low-micromolar concentrations of Mn2+ well below the substrate concentration (0.5 mM), in no case can Mg2+ replace Mn2+; required. Concentration of MnCl2 giving the half-maximal rate is 0.001-0.004 mM. Mn2+ cannot be replaced by Mg2+
Rattus norvegicus
additional information
Mg2+ cannot replace Mn2+
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Rattus norvegicus
38000
-
SDS-PAGE
Rattus norvegicus
39360
-
calculated from amino acid sequence
Rattus norvegicus
40000
-
1 * 39360, calculated, 1 * 40000, SDS-PAGE, enzyme plus N-terminal extension GPLGSPNS left after proteolysis of recombinant fusion protein
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione-Sepharose column chromatography; recombinant GSt-fusion protein, in-column proteolysis of fusion
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
additional information
preferential expression in immune cells
Mus musculus
-
skeletal muscle
-
Rattus norvegicus
-
spleen
ADPRibase-Mn activity in spleen is 2.5-5fold higher than in liver and muscle
Rattus norvegicus
-
splenocyte
-
Rattus norvegicus
-
thymus
ADPRibase-Mn activity in thymus is 2.5-5fold higher than in liver and muscle
Rattus norvegicus
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 1 mM EDTA, stable for months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
27% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
?
-
-
-
?
ADP + H2O
26% of the activity with ADP-ribose
690823
Rattus norvegicus
AMP + phosphate
-
-
-
?
ADP-ribose + H2O
-
690823
Rattus norvegicus
AMP + D-ribose 5-phosphate
-
-
-
?
ADP-ribose + H2O
100% activity at 0.5 mM substrate
690823
Rattus norvegicus
AMP + D-ribose 5-phosphate
-
-
-
?
CDP-choline + H2O
156% of the activity with ADP-ribose
690823
Rattus norvegicus
CMP + phosphocholine
-
-
-
?
CDP-choline + H2O
140% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
CMP + phosphocholine
-
-
-
?
CDP-ethanolamine + H2O
84% of the activity with ADP-ribose
690823
Rattus norvegicus
CMP + phosphoethanolamine
-
-
-
?
CDP-ethanolamine + H2O
89% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
?
-
-
-
?
CDP-glycerol + H2O
103% of the activity with ADP-ribose
690823
Rattus norvegicus
CMP + phosphoglycerol
-
-
-
?
CDP-glycerol + H2O
89% activity at 0.5 mM substrate compared to ADP-ribose
690823
Rattus norvegicus
?
-
-
-
?
additional information
ADP-glucose, UDP-glucose, CDP-glucose, CDP, CMP, and AMP are not hydrolysed
690823
Rattus norvegicus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 30000, gel filtration; 1 * 39360, calculated, 1 * 40000, SDS-PAGE, enzyme plus N-terminal extension GPLGSPNS left after proteolysis of recombinant fusion protein
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4
-
ADP
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
12.8
-
ADP-ribose
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 01 mM MnCl2, at 37°C
Rattus norvegicus
28.1
-
CDP-glycerol
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
28.5
-
CDP-choline
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
41.3
-
CDP-ethanolamine
pH 7.5, 37°C; recombinant enzyme, in 50 mM Tris-HCl (pH 7.5), 0.1 mM MnCl2, at 37°C
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
8
activity towards ADP- and CDP-alcohols
Rattus norvegicus
8
8.5
; ADP-ribose hydrolase activity
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
-
Rattus norvegicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
the activities towards ADP and CDP-alcohols are highest at pH 6-8 and decrease abruptly at higher pH
Rattus norvegicus
Other publictions for EC 3.6.1.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735009
Cabezas
Molecular bases of catalysis a ...
Homo sapiens
PLoS ONE
10
e0118680
2015
-
-
-
1
13
-
-
42
-
-
-
-
-
7
-
-
-
-
-
1
-
-
4
-
-
-
-
44
-
-
-
-
-
-
-
-
-
-
-
1
13
-
-
-
-
42
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
44
-
-
-
-
-
-
-
-
54
54
735057
Rodrigues
Characterization of Danio reri ...
Danio rerio
PLoS ONE
7
e42249
2012
-
-
-
1
1
-
-
13
-
2
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
13
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
13
-
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
13
1
2
-
-
-
-
-
-
13
13
697946
Canales
Hydrolysis of the phosphoanhyd ...
Rattus norvegicus
FEBS Lett.
583
1593-1598
2009
-
-
-
1
-
-
-
1
-
1
-
3
-
3
-
-
-
-
-
-
1
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
1
-
5
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
690823
Canales
Mn2+-dependent ADP-ribose/CDP- ...
Mus musculus, Rattus norvegicus
Biochem. J.
413
103-113
2008
-
-
1
1
-
-
2
5
-
2
4
-
-
6
-
-
1
-
-
7
-
1
11
1
-
-
-
5
2
1
1
-
-
-
-
-
-
1
-
1
-
-
-
2
-
5
-
2
4
-
-
-
-
1
-
7
-
1
11
1
-
-
-
5
2
1
1
-
-
-
-
-
-
-
209868
Canales
Rat liver nucleoside diphospho ...
Rattus norvegicus
Biochim. Biophys. Acta
1246
167-177
1995
-
-
-
-
-
-
-
1
-
2
1
-
-
1
-
-
1
-
-
1
1
-
10
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
1
-
1
1
-
10
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-