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Literature summary for 3.6.1.52 extracted from
- The g5R (D250) gene of African swine fever virus encodes a Nudix hydrolase that preferentially degrades diphosphoinositol polyphosphates (2002), J. Virol., 76, 1415-1421.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | African swine fever virus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
diphosphoinositol pentakisphosphate | pH 7.2, at 37ºC | African swine fever virus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation is required, pH 7.2, at 37ºC | African swine fever virus | |
| Mn2+ | divalent cation is required, 3 mM, pH 7.2, at 37ºC, 50% higher activity than Mg2+ at 10 to 15 mM | African swine fever virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
recombinant enzyme, SDS-PAGE | African swine fever virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphoinositol polyphosphate + H2O | African swine fever virus | regulation of stage of viral morphogenesis involving diphosphoinositol polyphosphate-mediated membrane trafficking | inositol polyphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| African swine fever virus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
African swine fever virus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, by nickel-nitrilotriacetic acid-agarose chromatography | African swine fever virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
African swine fever virus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphoinositol polyphosphate + H2O | regulation of stage of viral morphogenesis involving diphosphoinositol polyphosphate-mediated membrane trafficking | African swine fever virus | inositol polyphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D250 | - |
African swine fever virus |
| diphosphoinositol polyphosphate phosphohydrolase | - |
African swine fever virus |
| DIPP | - |
African swine fever virus |
| g5Rp | - |
African swine fever virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
African swine fever virus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity is 5% of that at pH 9.5 | African swine fever virus |
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Release 2023.1 (January 2023)
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