Literature summary for 3.6.1.52 extracted from

Yang, X.; Safrany, S.T.; Shears, S.B.
Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates (1999), J. Biol. Chem., 274, 35434-35440.

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Protein Variants

Protein Variants	Comment	Organism
E66Q	no significant activity towards diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate, activity with diadenosine 5',5'''-P1,P6-hexaphosphate is 2% of the activity of the wild-type enzyme	Homo sapiens
E70Q	inactive against dATP,diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate	Homo sapiens
F84Y	hydrolysis of bisdiphosphoinositol tetrakisphosphate at 63% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 75% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced	Homo sapiens
G50A	hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme	Homo sapiens
G52A	hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme	Homo sapiens
G72A	catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%	Homo sapiens
G75A	catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%	Homo sapiens
G78A	catalytic activity and CD spectrum is not significantly different from that of the wild.type enzyme	Homo sapiens
G82A	mutation promotes some unraveling of the normal secondary structure	Homo sapiens
H91L	hydrolysis of bisdiphosphoinositol tetrakisphosphate at 4% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 26% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	recombinant enzyme	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bisdiphosphoinositol tetrakisphosphate + H2O	-	Homo sapiens	? + phosphate	-	?
diadenosine 5',5''-P1,P6-hexaphosphate + H2O	-	Homo sapiens	?	-	?
diphosphoinositol pentakisphosphate + H2O	-	Homo sapiens	inositol hexakisphosphate + phosphate	-	?

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Release 2023.1 (January 2023)