Cloned (Comment) | Organism |
---|---|
TaAPY1, gene IDs TraesCS4A01G131300.1, TraesCS4B01G173300.1, and TraesCS4D01G175400.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY2, gene IDs TraesCS2A01G102100.1, TraesCS2B01G119200.1, and TraesCS2D01G101500.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY3-1, gene IDs TraesCS5A01G532000.1, TraesCS4B01G363700.1, and TraesCS4D01G357100.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY3-2, gene IDs TraesCS5A01G547700.1, TraesCS4B01G381600.1, and TraesCS4D01G357100.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY3-3, gene IDs TraesCS7A01G160900.1, TraesCS2B01G025000.1, and TraesCS2D01G020200.2, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY3-4, gene IDs TraesCS7B01G004400.2, TraesCS7D01G100000.1, and TraesCSU01G095000.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY5, gene IDs TraesCS6A01G105900.1, TraesCS7B01G178800.1, and TraesCS7D01G280900.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY6, gene IDs TraesCS6A01G105900.2, TraesCS6B01G135200.1, and TraesCS6D01G094400.2, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
TaAPY7, gene IDs TraesCS1A01G288900.1, TraesCS1B01G298200.1, and TraesCS1D01G287900.1, three different gene copies, sequence analysis and comparisons. Gene structure and conserved motif analysis of the APY genes in wheat, overview | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
ATP | TaAPY3-1, 37°C, pH 5.5, 8 mM Ca2+ | Triticum aestivum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Triticum aestivum | |
Mg2+ | required | Triticum aestivum | |
Mg2+ | activates | Triticum aestivum | |
additional information | Ca2+ is the most effective cofactor. The preference is in desecending order: Ca2+, Mg2+, Zn2+. Apyrase activity is dependent on ions as cofactors | Triticum aestivum | |
Zn2+ | activates | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 H2O | Triticum aestivum | - |
AMP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | specifically, TaAPY3-4 exhibits high expression level both in the leaf and root under cadmium stress | Triticum aestivum | - |
root | - |
Triticum aestivum | - |
seedling | gene expression pattern of the APYs in wheat seedlings in response to abiotic and biotic stresses, overview | Triticum aestivum | - |
shoot | - |
Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 H2O | - |
Triticum aestivum | AMP + 2 phosphate | - |
? | |
additional information | APY3-1 exhibits slightly lower enzymatic activity when degrading the ADP compared with ATP, but has very low activity during the degradation of TTP, GTP, and CTP, suggesting that TaAPY3-1 has a high substrate specificity | Triticum aestivum | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 36015-54540, TaAPY6, sequence calculation | Triticum aestivum |
? | x * 46446-49036, TaAPY3-2, sequence calculation | Triticum aestivum |
? | x * 46720-47258, TaAPY3-4, sequence calculation | Triticum aestivum |
? | x * 48910-50034, TaAPY2, sequence calculation | Triticum aestivum |
? | x * 49178-49979, TaAPY3-3, sequence calculation | Triticum aestivum |
? | x * 49209-54652, TaAPY5, sequence calculation | Triticum aestivum |
? | x * 49471-49555, TaAPY3-1, sequence calculation | Triticum aestivum |
? | x * 52225-52261, TaAPY1, sequence calculation | Triticum aestivum |
? | x * 77472-77557, TaAPY7, sequence calculation | Triticum aestivum |
More | three-dimensional structure, overview | Triticum aestivum |
More | TaAPY1, three-dimensional structure, overview | Triticum aestivum |
More | TaAPY2, three-dimensional structure, overview | Triticum aestivum |
More | TaAPY3-1 three-dimensional structure, overview | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
TaAPY1 | - |
Triticum aestivum |
TaAPY2 | - |
Triticum aestivum |
TaAPY3-1 | - |
Triticum aestivum |
TaAPY3-2 | - |
Triticum aestivum |
TaAPY3-3 | - |
Triticum aestivum |
TaAPY3-4 | - |
Triticum aestivum |
TaAPY5 | - |
Triticum aestivum |
TaAPY6 | - |
Triticum aestivum |
TaAPY7 | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Triticum aestivum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 52 | TaAPY3-1, activity range | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | - |
Triticum aestivum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum aestivum | TaAPY1, sequence calculation | 6.34 | 5.93 |
Triticum aestivum | TaAPY3-3, sequence calculation | 7.07 | 5.96 |
Triticum aestivum | TaAPY3-1, sequence calculation | 6.36 | 6.05 |
Triticum aestivum | TaAPY3-2, sequence calculation | 8.89 | 6.06 |
Triticum aestivum | TaAPY2, sequence calculation | 7.04 | 6.68 |
Triticum aestivum | TaAPY5, sequence calculation | 8.96 | 8.02 |
Triticum aestivum | TaAPY6, sequence calculation | 9.05 | 8.13 |
Triticum aestivum | TaAPY7, sequence calculation | 9.2 | 9.19 |
Triticum aestivum | sequence calculation | 9.4 | 9.29 |
Organism | Comment | Expression |
---|---|---|
Triticum aestivum | gene expression pattern of the APYs in wheat seedlings in response to abiotic and biotic stresses, overview | additional information |
Triticum aestivum | mannitol treatment is used to produce an artificial drought stress condition in the wheat seedlings. All the TaAPYs are upregulated within 24 h | up |
Triticum aestivum | mannitol treatment is used to produce an artificial drought stress condition in the wheat seedlings. All the TaAPYs are upregulated within 24 h, among which, TaAPY1, TaAPY3-4, and TaAPY6 reach an extremely high expression level in the leaves | up |
Triticum aestivum | mannitol treatment is used to produce an artificial drought stress condition in the wheat seedlings. All the TaAPYs are upregulated within 24 h, among which, TaAPY1, TaAPY3-4, and TaAPY6 reach an extremely high expression level in the leaves. TaAPY6 exhibits a significant upregulation at 6 h under cadmium stress | up |
Triticum aestivum | specifically, TaAPY3-4 exhibits high expression level both in the leaf and root under cadmium stress | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the NTPDase family | Triticum aestivum |
physiological function | apyrases, which directly regulate intra- and extracellular ATP homeostasis, play a pivotal role in the regulation of various stress adaptations in mammals, bacteria and plants | Triticum aestivum |