Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | oligomycin (an inhibitor of mitochondrial ATPases), orthovanadate (an inhibitor of transport ATPases, acid phosphatases, and phosphotyrosine phosphatases), Ap5A (an inhibitor of adenylate kinase [AK]), ouabain (a classic inhibitor of Na+-ATPase and K+-ATPase), NEM (Ca2+-ATPases and Mg2+-ATPases, AK and sulfhydryl group modifier), levamisole (inhibitor of alkaline phosphatase), tetramisole (inhibitor of alkaline phosphatase), and sodium azide (inhibitor of mitochondrial ATPases) below 10 mM do not show an inhibitory effect on ATP and ADP hydrolysis by E-NTPDase | Mus musculus | |
Sodium azide | - |
Mus musculus | |
Sodium fluoride | - |
Mus musculus | |
suramin | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
ATP | pH 8.5, 37°C | Mus musculus | |
0.66 | - |
ADP | pH 8.5, 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, activates at 1.5 mM | Mus musculus | |
Mg2+ | activates at 1.5 mM | Mus musculus | |
additional information | Ca2+-ATP and Ca2+-ADP hydrolysis are superior to hydrolysis with Mg2+-ATP and Mg2+-ADP as substrates | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Mus musculus | - |
AMP + phosphate | - |
? | |
ADP + H2O | Mus musculus BALB/c | - |
AMP + phosphate | - |
? | |
ATP + H2O | Mus musculus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Mus musculus BALB/c | - |
ADP + phosphate | - |
? | |
GDP + H2O | Mus musculus | - |
GMP + phosphate | - |
? | |
GTP + H2O | Mus musculus | - |
GDP + phosphate | - |
? | |
GTP + H2O | Mus musculus BALB/c | - |
GDP + phosphate | - |
? | |
UDP + H2O | Mus musculus | - |
UMP + phosphate | - |
? | |
UTP + H2O | Mus musculus | - |
UDP + phosphate | - |
? | |
UTP + H2O | Mus musculus BALB/c | - |
UDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P55772 | - |
- |
Mus musculus BALB/c | P55772 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte | - |
Mus musculus | - |
macrophage | - |
Mus musculus | - |
peritoneal cavity | - |
Mus musculus | - |
T-lymphocyte | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1208 | - |
substrate ADP, pH 8.5, 37°C | Mus musculus |
0.1364 | - |
substrate ATP, pH 8.5, 37°C | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Mus musculus | AMP + phosphate | - |
? | |
ADP + H2O | - |
Mus musculus BALB/c | AMP + phosphate | - |
? | |
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Mus musculus BALB/c | ADP + phosphate | - |
? | |
GDP + H2O | - |
Mus musculus | GMP + phosphate | - |
? | |
GTP + H2O | - |
Mus musculus | GDP + phosphate | - |
? | |
GTP + H2O | - |
Mus musculus BALB/c | GDP + phosphate | - |
? | |
additional information | adenine nucleotides are the best substrates. The other nucleotides (GTP, UTP, GDP, and UDP) are also hydrolyzed when added to the reaction instead of ATP or ADP, which demonstrates a broad substrate specificity for E-NTPDase expressed on the surface of peritoneal cavity cells | Mus musculus | ? | - |
- |
|
additional information | adenine nucleotides are the best substrates. The other nucleotides (GTP, UTP, GDP, and UDP) are also hydrolyzed when added to the reaction instead of ATP or ADP, which demonstrates a broad substrate specificity for E-NTPDase expressed on the surface of peritoneal cavity cells | Mus musculus BALB/c | ? | - |
- |
|
UDP + H2O | - |
Mus musculus | UMP + phosphate | - |
? | |
UTP + H2O | - |
Mus musculus | UDP + phosphate | - |
? | |
UTP + H2O | - |
Mus musculus BALB/c | UDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
E-NTPDase | - |
Mus musculus |
ectonucleoside triphosphate diphosphohydrolase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mus musculus |