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Literature summary for 3.6.1.5 extracted from

  • Endo, S.; Kato, R.; Sawada, K.; Hattori, T.
    Two-dimensional array ATP/ADP sensitive image sensor with a uniform distribution of chemically immobilized apyrase (2018), Bull. Chem. Soc. JPN, 91, 304-310 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ required Solanum tuberosum
Mg2+ required Solanum tuberosum

Application

Application Comment Organism
analysis usage of the enzyme in a two-dimensional array ATP/ADP sensitive image sensor, with a uniform distribution of chemically immobilized apyrase (via two different methods (3-APTES and CEST)). The enzyme sensor changes ATP into AMP, and the concentration of ATP reduces with time. The time-dependent profile of the potential response is different from the common ion sensor on which the concentration of the analyte ion is constant at an equilibrium state. Two ways of the measurement are used: 1. the addition measurement and 2. the steady measurement, overview Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
additional information development of a two-dimensional array ATP/ADP sensitive image sensor with a uniform distribution of chemically immobilized apyrase, immobilization via the two different methods 3-APTES and CEST, analysis method evaluation and optimization, overview. The surface of the ATP image sensor with 3-APTES method is polluted with the precipitation and shows heterogeneity. The ATP image sensor with CEST method shows a clear surface as well as that before the immobilization of apyrase. Potential disributions, and durability analysis Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Solanum tuberosum
-
AMP + phosphate
-
?
ATP + H2O Solanum tuberosum
-
ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum P80595
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
Solanum tuberosum AMP + phosphate
-
?
ATP + H2O
-
Solanum tuberosum ADP + phosphate
-
?