Cloned (Comment) | Organism |
---|---|
expression of the full-length protein and a soluble form without the transmembrane domain near the N-terminus in HEK-293 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 10 mM, 42% inhibition | Arabidopsis thaliana | |
Cu2+ | 1 mM, about 3fold inhibition | Arabidopsis thaliana | |
fluoride | 10 mM, 27% inhibition | Arabidopsis thaliana | |
vanadate | 1 mM, 54% inhibition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0597 | - |
GDP | pH 6.5, 25°C | Arabidopsis thaliana | |
0.0744 | - |
UDP | pH 6.5, 25°C | Arabidopsis thaliana | |
0.166 | - |
IDP | pH 6.5, 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Arabidopsis thaliana | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, about 3fold stimulation. Ca2+ and Mn2+ are best activators | Arabidopsis thaliana | |
Mg2+ | 1 mM, 2.5fold stimulation | Arabidopsis thaliana | |
Mn2+ | 1 mM, about 3fold stimulation. Ca2+ and Mn2+ are best activators | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, recombinant protein with His-tag, SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SQG2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.7 | - |
recombinant protein lacking transmembrane domain, pH 6.5, 25°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | - |
Arabidopsis thaliana | GMP + phosphate | - |
? | |
IDP + H2O | - |
Arabidopsis thaliana | IMP + phosphate | - |
? | |
additional information | no substrate: ADP | Arabidopsis thaliana | ? | - |
? | |
UDP + H2O | - |
Arabidopsis thaliana | UMP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant protein with His-tag, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
APY1 | - |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | broad | Arabidopsis thaliana |