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Literature summary for 3.6.1.5 extracted from

  • Okuhata, R.; Otsuka, Y.; Tsuchiya, T.; Kanzawa, N.
    Mutagenesis of apyrase conserved region 1 alters the nucleotide substrate specificity (2013), Plant Signal. Behav., 8, e24131.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mimosa pudica

Protein Variants

Protein Variants Comment Organism
A75S the Km value for ADP of the isoform APY2 mutant is decreased compared to the wild type enzyme, while the Km value for ATP is substantially increased. The mutant shows higher specific acitivity for ADP than ATP Mimosa pudica
S63A the ratio of the velocity of ATP/ADP hydrolysis is higher for the isoform MP67 mutant (approximately 1) compared to the wild type enzyme Mimosa pudica

Organism

Organism UniProt Comment Textmining
Mimosa pudica G1UFQ9 isoform MP67
-
Mimosa pudica G1UFR4 isoform APY2
-

Purification (Commentary)

Purification (Comment) Organism
His-Select nickel affinity column chromatography Mimosa pudica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O isoform MP67 demonstrates substantially higher substrate specificity for ADP than for ATP Mimosa pudica ?
-
 ?
ATP + 2 H2O isoform APY2 demonstrates slightly higher substrate specificity for ATP than for ADP Mimosa pudica AMP + 2 phosphate
-
 ?

Synonyms

Synonyms Comment Organism
APY2 isoform Mimosa pudica
MP67 isoform Mimosa pudica
NTPDase
-
 Mimosa pudica
nucleotide triphosphate diphosphohydrolase
-
 Mimosa pudica