Any feedback?
Literature summary for 3.6.1.5 extracted from
- The ATP/ADP substrate specificity switch between Toxoplasma gondii NTPDase1 and NTPDase3 is caused by an altered mode of binding of the substrate base (2013), ChemBioChem, 14, 2292-2300.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with (beta,gamma)-imido-ATP in the presence of Mg2+ ions, hanging drop vapor diffusion method, using Tris (pH 8.2-8.8, 100 mM), MgCl2 (50 mM), and pentaerythritol propoxylate (17/8 PO/OH, 23-25%, (v/v)) | Toxoplasma gondii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E493G | the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme | Toxoplasma gondii |
| R492G | the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme | Toxoplasma gondii |
| R492G/E493G | the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme | Toxoplasma gondii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.084 | - |
ATP | mutant enzyme E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii |  |
| 0.12 | - |
ATP | mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 2.5 | 3 | ATP | mutant enzyme R492G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 3.4 | - |
ATP | wild type enzyme, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Toxoplasma gondii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2 H2O | Toxoplasma gondii | - |
AMP + 2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | Q27895 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2 H2O | - |
Toxoplasma gondii | AMP + 2 phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTPDase1 | - |
Toxoplasma gondii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 280 | - |
ATP | wild type enzyme, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 1230 | - |
ATP | mutant enzyme R492G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 1410 | - |
ATP | mutant enzyme E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 2200 | - |
ATP | mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 81 | - |
ATP | wild type enzyme, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 490 | - |
ATP | mutant enzyme R492G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 17000 | - |
ATP | mutant enzyme E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
| 19000 | - |
ATP | mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
