Crystallization (Comment) | Organism |
---|---|
in complex with (beta,gamma)-imido-ATP in the presence of Mg2+ ions, hanging drop vapor diffusion method, using Tris (pH 8.2-8.8, 100 mM), MgCl2 (50 mM), and pentaerythritol propoxylate (17/8 PO/OH, 23-25%, (v/v)) | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
E493G | the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme | Toxoplasma gondii |
R492G | the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme | Toxoplasma gondii |
R492G/E493G | the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
ATP | mutant enzyme E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
0.12 | - |
ATP | mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
2.5 | 3 | ATP | mutant enzyme R492G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
3.4 | - |
ATP | wild type enzyme, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 H2O | Toxoplasma gondii | - |
AMP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q27895 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 H2O | - |
Toxoplasma gondii | AMP + 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NTPDase1 | - |
Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
280 | - |
ATP | wild type enzyme, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
1230 | - |
ATP | mutant enzyme R492G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
1410 | - |
ATP | mutant enzyme E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
2200 | - |
ATP | mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
81 | - |
ATP | wild type enzyme, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
490 | - |
ATP | mutant enzyme R492G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
17000 | - |
ATP | mutant enzyme E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii | |
19000 | - |
ATP | mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication | Toxoplasma gondii |