Cloned (Comment) | Organism |
---|---|
MP67 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli mainly in inclusion bodies | Mimosa pudica |
MpAPY2 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli | Mimosa pudica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
erythrosine B | does not affect the ATPase activity but inhibits ADPase activity of MP67 to a minor extent | Mimosa pudica | |
additional information | recombinant MpAPY2 shows no significant effect in the presence of vanadate | Mimosa pudica | |
Sodium fluoride | inhibits the ADPase activity rather than the ATPase activity of MP67 | Mimosa pudica | |
sodium orthovanadate | has a strong inhibitory effect on ATPase activity and a weaker effect on ADPase activity of MP67 | Mimosa pudica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Mimosa pudica | |
0.041 | - |
ADP | pH 7.5, 25°C | Mimosa pudica | |
0.169 | - |
ATP | pH 7.5, 25°C | Mimosa pudica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Mimosa pudica | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Mimosa pudica |
67000 | - |
x * 67000, SDS-PAGE | Mimosa pudica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Mimosa pudica | - |
AMP + phosphate | - |
? | |
ATP + 2 H2O | Mimosa pudica | - |
AMP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mimosa pudica | - |
- |
- |
Mimosa pudica | G1UFQ9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | MP67 is glycosylated | Mimosa pudica |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and concanavalin A affinity chromatography, recombinant enzyme from Escherichia coli | Mimosa pudica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mimosa pudica | - |
mesophyll | - |
Mimosa pudica | - |
additional information | MP67 is ubiquitously distributed in various tissues, most abundantly in leaves | Mimosa pudica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.8 | - |
substrate ATP, purified enzyme, pH 7.5, 25°C | Mimosa pudica |
12.8 | - |
substrate ATP, purified MP67, pH 7.5, 25°C | Mimosa pudica |
1140 | - |
substrate ADP, purified enzyme, pH 7.5, 25°C | Mimosa pudica |
1140 | - |
substrate ADP, purified MP67, pH 7.5, 25°C | Mimosa pudica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Mimosa pudica | AMP + phosphate | - |
? | |
ATP + 2 H2O | - |
Mimosa pudica | AMP + 2 phosphate | - |
? | |
additional information | the purified MP67 shows extremely high substrate specificity toward ADP in the presence of Ca2+ | Mimosa pudica | ? | - |
? | |
additional information | the recombinant MpAPY2 hydrolyzes ATP and ADP to the same extent | Mimosa pudica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Mimosa pudica |
? | x * 67000, SDS-PAGE | Mimosa pudica |
Synonyms | Comment | Organism |
---|---|---|
APY2-type apyrase | - |
Mimosa pudica |
ecto-apyrase | - |
Mimosa pudica |
MP67 | - |
Mimosa pudica |
MpAPY2 | - |
Mimosa pudica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mimosa pudica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mimosa pudica |