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Literature summary for 3.6.1.5 extracted from

  • Santos, R.F.; Possa, M.A.; Bastos, M.S.; Guedes, P.M.; Almeida, M.R.; Demarco, R.; Verjovski-Almeida, S.; Bahia, M.T.; Fietto, J.L.
    Influence of Ecto-nucleoside triphosphate diphosphohydrolase activity on Trypanosoma cruzi infectivity and virulence (2009), PLoS Negl. Trop. Dis., 3, e387.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.5

Application

Application Comment Organism
drug development Ecto-NTPDase1 is a target candidate in chemotherapy of Chagas disease Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
6-N,N-diethyl-beta,gamma-dibromomethylene-D-adenosine-5'-triphosphate i.e. ARL 67156, a selective inhibitor of Ecto-ATPase, shows 30% inhibition of Ecto-ATPDase activities and 50% inhibition of trypomastigotes infectivity in vivo at 0.5 mM and 0.3 mM, respectively Trypanosoma cruzi
gadolinium GdCl3, an Ecto-ATPDase inhibitor, shows 30% inhibition of Ecto-ATPDase activities and 60% inhibition of trypomastigotes infectivity in vivo at 0.3 mM and 0.5 mM, respectively Trypanosoma cruzi
additional information inhibition of Ecto-ATPDase by ant-Ecto-NTPDase-anti-serum, inhibition of Ecto-ATPDase activities and of trypomastigotes infectivity in vivo Trypanosoma cruzi
suramin an Ecto-ATPDase inhibitor, shows 60% inhibition of Ecto-ATPDase activities and 75% inhibition of trypomastigotes infectivity in vivo at 0.1 mM and 0.5 mM, respectively Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + 2 H2O Trypanosoma cruzi
-
 adenosine + 2 phosphate
-
 ?
ATP + 2 H2O Trypanosoma cruzi
-
 AMP + 2 phosphate
-
 ?
additional information Trypanosoma cruzi Ecto-NTPDase1 is required in the infection process of trypanosomes into mammalian cells, overview. Ecto-NTPDase act as facilitators of infection and virulence in vitro and in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q6QHT8 wild-type strains Y, CL, and Be-62, and hybrid strain and CL Brener
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
trypomastigote sequential sub-cultivation in mammalian cells, at third to fourth passages most of the cell-derived trypomastigotes cannot penetrate mammalian cells and have differentiated into amastigote-like parasites that exhibit 3-4fold lower levels of Ecto-NTPDase activities Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.238
-
 ADPase activity, purified recombinant His-tagged enzyme Trypanosoma cruzi
0.4
-
 ATPase activity, purified recombinant His-tagged enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 2 H2O
-
 Trypanosoma cruzi adenosine + 2 phosphate
-
 ?
ATP + 2 H2O
-
 Trypanosoma cruzi AMP + 2 phosphate
-
 ?
additional information Ecto-NTPDase1 is required in the infection process of trypanosomes into mammalian cells, overview. Ecto-NTPDase act as facilitators of infection and virulence in vitro and in vivo Trypanosoma cruzi ?
-
 ?

Synonyms

Synonyms Comment Organism
Apyrase
-
 Trypanosoma cruzi
ecto-ATPDase
-
 Trypanosoma cruzi
ecto-NTPDase
-
 Trypanosoma cruzi
Ecto-NTPDase1
-
 Trypanosoma cruzi
ecto-nucleoside-triphosphate-diphosphohydrolase
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Trypanosoma cruzi