Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of isozyme I, phylogenetic analysis, expression of isozyme I as secreted protein in Pichia pastoris strain GS115 | Ornithodoros savignyi |
DNA and amino acid sequence determination and analysis of isozyme II, phylogenetic analysis | Ornithodoros savignyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, recombinant, secreted isozyme I, SDS-PAGE | Ornithodoros savignyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ornithodoros savignyi | salivary apyrases are nucleotide-metabolising enzymes that blood-feeding parasites utilise for modulation of extracellular nucleotides to prevent platelet activation and aggregation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ornithodoros savignyi | A7LH73 | isozyme I; collected from the Upington region in the Western Cape, South Africa by sifting of sand, isozyme I | - |
Ornithodoros savignyi | A7LH74 | isozyme II; collected from the Upington region in the Western Cape, South Africa by sifting of sand, isozyme II | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | predicted site of cleavage for the mature protein of the 50-nucleotidase isoforms, mass spectrometry | Ornithodoros savignyi |
Purification (Comment) | Organism |
---|---|
native enzyme from salivary gland by anion exchange chromatography, ultrafiltration, and cation exchange chromatography | Ornithodoros savignyi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | - |
Ornithodoros savignyi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | salivary apyrases are nucleotide-metabolising enzymes that blood-feeding parasites utilise for modulation of extracellular nucleotides to prevent platelet activation and aggregation | Ornithodoros savignyi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, recombinant, secreted isozyme I, SDS-PAGE | Ornithodoros savignyi |
More | comparisons of predicted isozyme tertiary structure, N-terminal Edman sequence analysis and mass spectrometry, overview | Ornithodoros savignyi |
More | comparisons of predicted isozyme tertiary structures, N-terminal Edman sequence analysis and mass spectrometry, overview | Ornithodoros savignyi |
Synonyms | Comment | Organism |
---|---|---|
5'-nucleotidase | - |
Ornithodoros savignyi |
ecto-5'-nucleotidase | - |
Ornithodoros savignyi |
More | the apyrase belongs to the 5'-nucleotidase family | Ornithodoros savignyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ornithodoros savignyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Ornithodoros savignyi |