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Literature summary for 3.6.1.5 extracted from

  • Chiang, W.C.; Knowles, A.F.
    Transmembrane domain interactions affect the stability of the extracellular domain of the human NTPDase 2 (2008), Arch. Biochem. Biophys., 472, 89-99.
    View publication on PubMed
Search for more literature for 3.6.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant hu-ck ACR1,5 chimeric enzymes in HEK-293 cells Gallus gallus
expression of mutant hu-ck ACR1,5 chimeric enzymes in HEK-293 cells, stable expression of soluble His-tagged secreted NTPDase2 extracellular domain in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a hu-ck ACR1,5 chimera in which the extracellular domain is anchored to the membrane by the two transmembrane domains of the chicken NTPDase8. The hu-ck ACR1,5 chimera is the first chimeric NTPDase reported that shows a resistance to membrane perturbation and substrate inactivation. The strengths of interaction of the respective transmembrane domain pairs of the human NTPDase2 and chicken NTPDase8 determine their different responses to membrane perturbation and substrate. The chimeric mutants all show highly reduced ATPase activities, overview. Catalysis at the active site in the extracellular domain of the hu-ck ACR1,5 chimera is no longer negatively affected by membrane perturbation in the lipid bilayer by detergent and temperature Homo sapiens
additional information construction of a hu-ck ACR1,5 chimera in which the extracellular domain of human NTPDase2 is anchored to the membrane by the two transmembrane domains of the chicken NTPDase8. The hu-ck ACR1,5 chimera is the first chimeric NTPDase reported that shows a resistance to membrane perturbation and substrate inactivation. The strength of interaction of the respective transmembrane domain pairs of the human NTPDase2 and chicken NTPDase8 determine their different responses to membrane perturbation and substrate. The chimeric mutants all show highly reduced ATPase activities, overview. Catalysis at the active site in the extracellular domain of the hu-ck ACR1,5 chimera is no longer negatively affected by membrane perturbation in the lipid bilayer by detergent and temperature Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
detergent NP-40 at low concentration inhibition of the membrane-bound enzyme, not of the soluble enzyme Homo sapiens
additional information the chicken enzyme does not show substrate inhibition Gallus gallus
additional information the enzyme shows substrate inhibition Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the enzyme contains two transmembrane domains and five apyrase conserved regions, ACRs. ACR1 is located near the N-terminal transmembrane domain, whereas ACR5 is located near the C-terminal transmembrane domain Homo sapiens 9986
-
membrane the enzyme contains two transmembrane domains and five apyrase conserved regions, ACRs. ACR1 is located near the N-terminal transmembrane domain, whereas ACR5 is located near the C-terminal transmembrane domain Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, required Homo sapiens
Mg2+ activates, required Homo sapiens
Mg2+ required, activates Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, recombinant soluble His-tagged secreted NTPDase2 extracellular domain, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2 H2O Homo sapiens
-
 AMP + 2 phosphate
-
 ?
ATP + 2 H2O Gallus gallus
-
 AMP + 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus O93295
-
-
Homo sapiens Q9Y5L3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged soluble NTPDase2 extracellular domain from HEK-293 cells by ammonium sulfate fractionation, ultrafiltration, nickel affinity chromatography, and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 recombinant chimeric mutant hu-ck ACR1,5 Homo sapiens
0.17
-
 recombinant chimeric mutant hu-ck ACR1,5 Gallus gallus
2.33
-
 purified recombinant wild-type enzyme Homo sapiens
1020
-
 purified recombinant extracellular domain, pH 7.5, in presence of Ca2+ Homo sapiens

Storage Stability

Storage Stability Organism
4°C, purified recombinant extracellular domain, stable for several weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2 H2O
-
 Homo sapiens AMP + 2 phosphate
-
 ?
ATP + 2 H2O
-
 Gallus gallus AMP + 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, recombinant soluble His-tagged secreted NTPDase2 extracellular domain, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
NTPDase 2
-
 Homo sapiens
NTPDase 8
-
 Gallus gallus
NTPDase2
-
 Homo sapiens
NTPDase8
-
 Gallus gallus
nucleoside triphosphate diphosphohydrolase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus
37
-
 native full-length enzyme Homo sapiens
62
-
 above, enzyme, recombinant soluble His-tagged secreted NTPDase2 extracellular domain Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 native full-length enzyme Homo sapiens
30 62 and above, recombinant soluble His-tagged secreted NTPDase2 extracellular domain Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens
7.5
-
 assay at Gallus gallus