Cloned (Comment) | Organism |
---|---|
expression of mutant hu-ck ACR1,5 chimeric enzymes in HEK-293 cells | Gallus gallus |
expression of mutant hu-ck ACR1,5 chimeric enzymes in HEK-293 cells, stable expression of soluble His-tagged secreted NTPDase2 extracellular domain in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a hu-ck ACR1,5 chimera in which the extracellular domain is anchored to the membrane by the two transmembrane domains of the chicken NTPDase8. The hu-ck ACR1,5 chimera is the first chimeric NTPDase reported that shows a resistance to membrane perturbation and substrate inactivation. The strengths of interaction of the respective transmembrane domain pairs of the human NTPDase2 and chicken NTPDase8 determine their different responses to membrane perturbation and substrate. The chimeric mutants all show highly reduced ATPase activities, overview. Catalysis at the active site in the extracellular domain of the hu-ck ACR1,5 chimera is no longer negatively affected by membrane perturbation in the lipid bilayer by detergent and temperature | Homo sapiens |
additional information | construction of a hu-ck ACR1,5 chimera in which the extracellular domain of human NTPDase2 is anchored to the membrane by the two transmembrane domains of the chicken NTPDase8. The hu-ck ACR1,5 chimera is the first chimeric NTPDase reported that shows a resistance to membrane perturbation and substrate inactivation. The strength of interaction of the respective transmembrane domain pairs of the human NTPDase2 and chicken NTPDase8 determine their different responses to membrane perturbation and substrate. The chimeric mutants all show highly reduced ATPase activities, overview. Catalysis at the active site in the extracellular domain of the hu-ck ACR1,5 chimera is no longer negatively affected by membrane perturbation in the lipid bilayer by detergent and temperature | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
detergent NP-40 | at low concentration inhibition of the membrane-bound enzyme, not of the soluble enzyme | Homo sapiens | |
additional information | the chicken enzyme does not show substrate inhibition | Gallus gallus | |
additional information | the enzyme shows substrate inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | the enzyme contains two transmembrane domains and five apyrase conserved regions, ACRs. ACR1 is located near the N-terminal transmembrane domain, whereas ACR5 is located near the C-terminal transmembrane domain | Homo sapiens | 9986 | - |
membrane | the enzyme contains two transmembrane domains and five apyrase conserved regions, ACRs. ACR1 is located near the N-terminal transmembrane domain, whereas ACR5 is located near the C-terminal transmembrane domain | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, required | Homo sapiens | |
Mg2+ | activates, required | Homo sapiens | |
Mg2+ | required, activates | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, recombinant soluble His-tagged secreted NTPDase2 extracellular domain, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 H2O | Homo sapiens | - |
AMP + 2 phosphate | - |
? | |
ATP + 2 H2O | Gallus gallus | - |
AMP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | O93295 | - |
- |
Homo sapiens | Q9Y5L3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged soluble NTPDase2 extracellular domain from HEK-293 cells by ammonium sulfate fractionation, ultrafiltration, nickel affinity chromatography, and gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.17 | - |
recombinant chimeric mutant hu-ck ACR1,5 | Homo sapiens |
0.17 | - |
recombinant chimeric mutant hu-ck ACR1,5 | Gallus gallus |
2.33 | - |
purified recombinant wild-type enzyme | Homo sapiens |
1020 | - |
purified recombinant extracellular domain, pH 7.5, in presence of Ca2+ | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, purified recombinant extracellular domain, stable for several weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2 H2O | - |
Homo sapiens | AMP + 2 phosphate | - |
? | |
ATP + 2 H2O | - |
Gallus gallus | AMP + 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, recombinant soluble His-tagged secreted NTPDase2 extracellular domain, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NTPDase 2 | - |
Homo sapiens |
NTPDase 8 | - |
Gallus gallus |
NTPDase2 | - |
Homo sapiens |
NTPDase8 | - |
Gallus gallus |
nucleoside triphosphate diphosphohydrolase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
37 | - |
native full-length enzyme | Homo sapiens |
62 | - |
above, enzyme, recombinant soluble His-tagged secreted NTPDase2 extracellular domain | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | native full-length enzyme | Homo sapiens |
30 | 62 | and above, recombinant soluble His-tagged secreted NTPDase2 extracellular domain | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Gallus gallus |