Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bafilomycin | 13% activation at 0.001 mM | Trypanosoma brucei brucei | |
Ouabain | 13% activation at 1 mM | Trypanosoma brucei brucei | |
Tartrate | 14% activation at 10 mM | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,4'-diisothiocyanostilbene 2,2'-disulfonate | DIDS, an impermeable inhibitor | Trypanosoma brucei brucei | |
4-nitrophenyl phosphate | 15% inhibition at 5 mM | Trypanosoma brucei brucei | |
5'-AMP | 11% inhibition at 5 mM | Trypanosoma brucei brucei | |
ADP | 50% inhibition at 5 mM | Trypanosoma brucei brucei | |
dipyridamole | 10% inhibition at 0.01 mM | Trypanosoma brucei brucei | |
Furosemide | 20% inhibition at 1 mM | Trypanosoma brucei brucei | |
additional information | no inhibition by oligomycin, ouabain, and molybdate | Trypanosoma brucei brucei | |
NaN3 | 58% inhibition at 10 mM | Trypanosoma brucei brucei | |
suramin | a P2 purinoreceptor antagonist, 50% inhibition at 0.05-0.5 mM | Trypanosoma brucei brucei | |
vanadate | 15% inhibition at 1 mM | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
ATP | pH 7.2, 30°C | Trypanosoma brucei brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Trypanosoma brucei brucei | |
Mg2+ | activates, most effect divalent cation, activity profile, overview | Trypanosoma brucei brucei | |
Mn2+ | activates, most effect divalent cation | Trypanosoma brucei brucei | |
Zn2+ | activates | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Trypanosoma brucei brucei | hydrolysis of extracellular ADP | AMP + phosphate | - |
? | |
AMP + H2O | Trypanosoma brucei brucei | hydrolysis of extracellular AMP, low activity | adenosine + phosphate | - |
? | |
ATP + H2O | Trypanosoma brucei brucei | hydrolysis of extracellular ATP | ADP + phosphate | - |
? | |
additional information | Trypanosoma brucei brucei | the enzyme plays a role in the salvage of purines from the extracellular medium in the organism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
integument | - |
Trypanosoma brucei brucei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Trypanosoma brucei brucei | AMP + phosphate | - |
? | |
ADP + H2O | hydrolysis of extracellular ADP | Trypanosoma brucei brucei | AMP + phosphate | - |
? | |
AMP + H2O | low activity | Trypanosoma brucei brucei | adenosine + phosphate | - |
? | |
AMP + H2O | hydrolysis of extracellular AMP, low activity | Trypanosoma brucei brucei | adenosine + phosphate | - |
? | |
ATP + H2O | - |
Trypanosoma brucei brucei | ADP + phosphate | - |
? | |
ATP + H2O | hydrolysis of extracellular ATP | Trypanosoma brucei brucei | ADP + phosphate | - |
? | |
CTP + H2O | - |
Trypanosoma brucei brucei | CDP + phosphate | - |
? | |
GTP + H2O | - |
Trypanosoma brucei brucei | GDP + phosphate | - |
? | |
ITP + H2O | best substrate | Trypanosoma brucei brucei | IDP + phosphate | - |
? | |
additional information | substrate specificity, overview | Trypanosoma brucei brucei | ? | - |
? | |
additional information | the enzyme plays a role in the salvage of purines from the extracellular medium in the organism | Trypanosoma brucei brucei | ? | - |
? | |
TTP + H2O | low activity | Trypanosoma brucei brucei | TDP + phosphate | - |
? | |
UTP + H2O | - |
Trypanosoma brucei brucei | UDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
E-NTPDase | - |
Trypanosoma brucei brucei |
ecto-ATPase | - |
Trypanosoma brucei brucei |
ecto-nucleoside triphosphate diphosphohydrolase | - |
Trypanosoma brucei brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trypanosoma brucei brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Trypanosoma brucei brucei |