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Literature summary for 3.6.1.5 extracted from

  • Sun, D.; McNicol, A.; James, A.A.; Peng, Z.
    Expression of functional recombinant mosquito salivary apyrase: a potential therapeutic platelet aggregation inhibitor (2006), Platelets, 17, 178-184.
    View publication on PubMed
Search for more literature for 3.6.1.5

Application

Application Comment Organism
medicine enzyme inhibits ADP- collagen- and thrombin-induced human platelet aggregation in dose-dependent manner, use as a therapeutic agent for inhibition of platelet-mediated thrombosis Aedes aegypti
pharmacology the enzyme may serve as a therapeutic agent for inhibition of platelet-mediated thrombosis Aedes aegypti

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Aedes aegypti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 ADP
-
 Aedes aegypti
0.0125
-
 ADP pH 7.5, 37°C Aedes aegypti
0.015
-
 ATP
-
 Aedes aegypti
0.015
-
 ATP pH 7.5, 37°C Aedes aegypti

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aedes aegypti
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Aedes aegypti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, SDS-PAGE Aedes aegypti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Aedes aegypti
-
 AMP + phosphate
-
 ?
ATP + H2O Aedes aegypti
-
 ADP + phosphate
-
 ?
additional information Aedes aegypti enzyme inhibits ADP-, collagen-, and thrombin-induced human platelet aggregation in dose-dependent manner ?
-
 ?
additional information Aedes aegypti apyrase, an ecto-enzyme with ADPase and ATPase activities, rapidly metabolizes ADP and ATP released from platelets and endothelial cells, thereby reducing platelet activation and recruitment. The recombinant apyrase inhibits ADP-, collagen- and thrombin-induced human platelet aggregation, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aedes aegypti
-
-
-
Aedes aegypti
-
 recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from insect Sf9 cells by anion exchange chromatography to homogeneity, large scale preparation Aedes aegypti

Source Tissue

Source Tissue Comment Organism Textmining
additional information apyrase is an ecto-enzyme Aedes aegypti
-
saliva
-
 Aedes aegypti
-
salivary gland enzyme synthesis Aedes aegypti
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Aedes aegypti AMP + phosphate
-
 ?
ATP + 2 H2O
-
 Aedes aegypti AMP + 2 phosphate
-
 ?
ATP + H2O
-
 Aedes aegypti ADP + phosphate
-
 ?
additional information enzyme inhibits ADP-, collagen-, and thrombin-induced human platelet aggregation in dose-dependent manner Aedes aegypti ?
-
 ?
additional information apyrase, an ecto-enzyme with ADPase and ATPase activities, rapidly metabolizes ADP and ATP released from platelets and endothelial cells, thereby reducing platelet activation and recruitment. The recombinant apyrase inhibits ADP-, collagen- and thrombin-induced human platelet aggregation, overview Aedes aegypti ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Aedes aegypti

Synonyms

Synonyms Comment Organism
Apyrase
-
 Aedes aegypti
ATP diphosphohyrolase
-
 Aedes aegypti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aedes aegypti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Aedes aegypti