Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-p-fluorosulfonylbenzoyladenosine | 50% inhibition at 2 mM | Sus scrofa | |
EGTA | - |
Sus scrofa | |
additional information | no inhibition by NaN3 | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ATP | - |
Sus scrofa | |
0.1 | - |
ADP | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | ectonucleotidase/ectoenzyme | Sus scrofa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ preferred to Mg2+ | Sus scrofa | |
Mg2+ | either Mg2+ or Ca2+ required for activity | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
highly glycosylated protein, N-linked oligosaccarides, immunoaffinity techniques, SDS-PAGE | Sus scrofa |
78000 | - |
immunoaffinity techniques, SDS-PAGE, ATPDase/cd39 | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Sus scrofa | - |
AMP + phosphate | - |
? | |
ATP + 2 H2O | Sus scrofa | - |
AMP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
pig | - |
Sus scrofa | - |
enzyme belongs to E-type ATPase/NTPDase family | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-linked oligosaccarides | Sus scrofa |
Purification (Comment) | Organism |
---|---|
purified from liver | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | - |
Sus scrofa | - |
kidney | ATPDase/cd39 detected by immunoblotting in vascular endothelium and smooth muscles (blood vessel walls) | Sus scrofa | - |
kidney | ATPDase detected by immunoblotting in luminal side of the ductular epithelium | Sus scrofa | - |
liver | ATPDase/cd39 detected by immunoblotting in vascular endothelium and smooth muscles (blood vessel walls) | Sus scrofa | - |
liver | ATPDase detected by immunoblotting in bile canaliculi of hepatocytes | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Sus scrofa | AMP + phosphate | - |
? | |
ATP + 2 H2O | - |
Sus scrofa | AMP + 2 phosphate | - |
? | |
CDP + H2O | - |
Sus scrofa | CMP + phosphate | - |
? | |
CTP + 2 H2O | - |
Sus scrofa | CMP + 2 phosphate | - |
? | |
GDP + H2O | - |
Sus scrofa | GMP + phosphate | - |
? | |
GTP + 2 H2O | - |
Sus scrofa | GMP + 2 phosphate | - |
? | |
IDP + H2O | - |
Sus scrofa | IMP + phosphate | - |
? | |
ITP + 2 H2O | - |
Sus scrofa | IMP + 2 phosphate | - |
? | |
additional information | neglible hydrolysis of AMP | Sus scrofa | ? | - |
? | |
TDP + H2O | - |
Sus scrofa | TMP + phosphate | - |
? | |
TTP + 2 H2O | - |
Sus scrofa | TMP + 2 phosphate | - |
? | |
UDP + H2O | - |
Sus scrofa | UMP + phosphate | - |
? | |
UTP + 2 H2O | - |
Sus scrofa | UMP + 2 phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nucleoside triphosphate diphosphohydrolase-1, NDPDase 1 | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | - |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | 50% activity at pH 6, no activity at pH 9.5 | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
5'-p-fluorosulfonylbenzoyladenosine | - |
Sus scrofa |