Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant UshA enzyme lacking signal peptide in Escherichia coli 5A-1, UshA lacking the signal peptide is an active cytoplasmic 5' nucleotidase which causes conditional lethality | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | carboxyterminal deletion which abolishes the 5' nucleotidase activity and deletion of the signal peptide | Escherichia coli |
additional information | deletion of the signal peptide, identical to the mature periplasmic enzyme, except for the presence of an N-terminal methionine residue, expressed in Escherichia coli 5A-1 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | enzyme lacking signal sequence except for that encoding the initiating methionine residue, expressed in Escherichia coli 5A-1 | Escherichia coli | 5737 | - |
periplasm | wild-type expressed in Escherichia coli TG1 and 5A-1 | Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 TG1 and K12 5A-1 strains | - |
Synonyms | Comment | Organism |
---|---|---|
UshA | bifunctional enzyme with UDP-sugar hydrolase and 5' nucleotidase activity | Escherichia coli |