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show all sequences of 3.6.1.44

Characterization of an oligosaccharide-pyrophosphodolichol pyrophosphatase activity in yeast

Belard, M.; Cacan, R.; Verbert,A.; Biochem. J. 255, 235-242 (1988)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
6fold increase at 0.5%
Saccharomyces cerevisiae
Triton X-114
7fold increase at 0.5%
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
bacitracin
80% inhibition at 10 mM
Saccharomyces cerevisiae
diphosphate
total inhibition at 10 mM
Saccharomyces cerevisiae
NAD+
total inhibition at 10 mM
Saccharomyces cerevisiae
phosphate
35% inhibition at 10 mM
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Saccharomyces cerevisiae
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
150% activity compared to control, 10 mM
Saccharomyces cerevisiae
Mg2+
113% activity compared to control, 10 mM
Saccharomyces cerevisiae
Mn2+
149.5% activity compared to control, 10 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oligosaccharide-diphosphodolichol + H2O
Saccharomyces cerevisiae
-
oligosaccharide phosphate + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
oligosaccharide-diphosphodolichol + H2O
-
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
oligosaccharide-diphosphodolichol + H2O
oligosaccharide-diphosphodolichol from mouse splenocytes
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
35
no activity at 0C
Saccharomyces cerevisiae
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
preincubation for 10 min, half-life at 62C 1 hour, activity lost after incubation at 70C
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
no activity above pH 9.0
Saccharomyces cerevisiae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
7.5
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
6fold increase at 0.5%
Saccharomyces cerevisiae
Triton X-114
7fold increase at 0.5%
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
bacitracin
80% inhibition at 10 mM
Saccharomyces cerevisiae
diphosphate
total inhibition at 10 mM
Saccharomyces cerevisiae
NAD+
total inhibition at 10 mM
Saccharomyces cerevisiae
phosphate
35% inhibition at 10 mM
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Saccharomyces cerevisiae
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
150% activity compared to control, 10 mM
Saccharomyces cerevisiae
Mg2+
113% activity compared to control, 10 mM
Saccharomyces cerevisiae
Mn2+
149.5% activity compared to control, 10 mM
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oligosaccharide-diphosphodolichol + H2O
Saccharomyces cerevisiae
-
oligosaccharide phosphate + dolichyl phosphate
-
Saccharomyces cerevisiae
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
oligosaccharide-diphosphodolichol + H2O
-
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
oligosaccharide-diphosphodolichol + H2O
oligosaccharide-diphosphodolichol from mouse splenocytes
289083
Saccharomyces cerevisiae
oligosaccharide phosphate + dolichyl phosphate
-
289083
Saccharomyces cerevisiae
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
35
no activity at 0C
Saccharomyces cerevisiae
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
preincubation for 10 min, half-life at 62C 1 hour, activity lost after incubation at 70C
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
no activity above pH 9.0
Saccharomyces cerevisiae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
7.5
-
Saccharomyces cerevisiae
Other publictions for EC 3.6.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
289083
Belard
Characterization of an oligosa ...
Saccharomyces cerevisiae
Biochem. J.
255
235-242
1988
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4
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2
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1
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