BRENDA - Enzyme Database show
show all sequences of 3.6.1.40

Guanosine 5'-triphosphate, 3'-diphosphate 5'-phosphohydrolase. Purification and substrate specificity

Hara, A.; Sy, J.; J. Biol. Chem. 258, 1678-1683 (1983)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
guanosine 5'-triphosphate,3'-diphosphate
-
Escherichia coli
0.13
-
Guanosine 5'-triphosphate,3'-monophosphate
-
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
monovalent cation required, NH4+ preferred over K+, Na+ ineffective
Escherichia coli
Mg2+
required, optimum activity above 1 mM
Escherichia coli
NH4+
monovalent cation required, NH4+ preferred over K+, Na+ ineffective
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
guanosine 5'-triphosphate,3'-diphosphate + H2O
Escherichia coli
involved in nutritional stress response via ppGpp
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
Escherichia coli
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
Escherichia coli JF1599
involved in nutritional stress response via ppGpp
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
Escherichia coli JF1599
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli JF1599
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.08
-
crude extract
Escherichia coli
1513
-
partially purified enzyme
Escherichia coli
Storage Stability
Storage Stability
Organism
unstable at 4°C, storable in liquid nitrogen
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
289072
Escherichia coli
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
involved in nutritional stress response via ppGpp
289072
Escherichia coli
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
289072
Escherichia coli JF1599
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli JF1599
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
involved in nutritional stress response via ppGpp
289072
Escherichia coli JF1599
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli JF1599
?
guanosine 5'-triphosphate,3'-monophosphate + H2O
-
289072
Escherichia coli
guanosine 5'-diphosphate,3'-monophosphate + phosphate
-
289072
Escherichia coli
?
guanosine 5'-triphosphate,3'-monophosphate + H2O
-
289072
Escherichia coli JF1599
guanosine 5'-diphosphate,3'-monophosphate + phosphate
-
289072
Escherichia coli JF1599
?
additional information
not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential
289072
Escherichia coli
?
-
-
-
-
additional information
not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential
289072
Escherichia coli JF1599
?
-
-
-
-
pppGpNp + H2O
cleavage of 5'-phosphate ends of RNA
289072
Escherichia coli
ppGpNp + phosphate
-
289072
Escherichia coli
?
pppGpNp + H2O
cleavage of 5'-phosphate ends of RNA
289072
Escherichia coli JF1599
ppGpNp + phosphate
-
289072
Escherichia coli JF1599
?
pppGpNp-RNA + H2O
cleavage of 5'-phosphate ends of RNA, poor substrate
289072
Escherichia coli
ppGpNp-RNA + phosphate
-
289072
Escherichia coli
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
optimum activity in borate buffer
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
guanosine 5'-triphosphate,3'-diphosphate
-
Escherichia coli
0.13
-
Guanosine 5'-triphosphate,3'-monophosphate
-
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
monovalent cation required, NH4+ preferred over K+, Na+ ineffective
Escherichia coli
Mg2+
required, optimum activity above 1 mM
Escherichia coli
NH4+
monovalent cation required, NH4+ preferred over K+, Na+ ineffective
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
140000
-
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
guanosine 5'-triphosphate,3'-diphosphate + H2O
Escherichia coli
involved in nutritional stress response via ppGpp
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
Escherichia coli
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
Escherichia coli JF1599
involved in nutritional stress response via ppGpp
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
Escherichia coli JF1599
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.08
-
crude extract
Escherichia coli
1513
-
partially purified enzyme
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
unstable at 4°C, storable in liquid nitrogen
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
289072
Escherichia coli
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
involved in nutritional stress response via ppGpp
289072
Escherichia coli
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
289072
Escherichia coli JF1599
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli JF1599
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
involved in nutritional stress response via ppGpp
289072
Escherichia coli JF1599
guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
289072
Escherichia coli JF1599
?
guanosine 5'-triphosphate,3'-monophosphate + H2O
-
289072
Escherichia coli
guanosine 5'-diphosphate,3'-monophosphate + phosphate
-
289072
Escherichia coli
?
guanosine 5'-triphosphate,3'-monophosphate + H2O
-
289072
Escherichia coli JF1599
guanosine 5'-diphosphate,3'-monophosphate + phosphate
-
289072
Escherichia coli JF1599
?
additional information
not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential
289072
Escherichia coli
?
-
-
-
-
additional information
not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential
289072
Escherichia coli JF1599
?
-
-
-
-
pppGpNp + H2O
cleavage of 5'-phosphate ends of RNA
289072
Escherichia coli
ppGpNp + phosphate
-
289072
Escherichia coli
?
pppGpNp + H2O
cleavage of 5'-phosphate ends of RNA
289072
Escherichia coli JF1599
ppGpNp + phosphate
-
289072
Escherichia coli JF1599
?
pppGpNp-RNA + H2O
cleavage of 5'-phosphate ends of RNA, poor substrate
289072
Escherichia coli
ppGpNp-RNA + phosphate
-
289072
Escherichia coli
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
optimum activity in borate buffer
Escherichia coli
Other publictions for EC 3.6.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735334
Malde
Functional characterization of ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Virulence
5
521-533
2014
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8
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1
2
-
-
-
688392
Kristensen
Structure of the PPX/GPPA phos ...
Aquifex aeolicus
J. Mol. Biol.
375
1469-1476
2008
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-
1
1
1
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1
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1
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3
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1
1
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2
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1
-
1
1
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1
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1
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1
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2
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654764
Kristensen
Structural characterization of ...
Aquifex aeolicus
Biochemistry
43
8894-8900
2004
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-
-
1
-
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-
-
1
-
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2
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1
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1
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-
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-
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-
209848
Keasling
Guanosine pentaphosphate phosp ...
Escherichia coli, Escherichia coli CA10
Proc. Natl. Acad. Sci. USA
90
7029-7033
1993
-
-
-
-
-
-
3
2
-
-
2
2
-
3
-
-
1
-
-
-
2
-
4
-
-
-
-
2
-
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-
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3
-
2
-
-
2
2
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-
-
1
-
-
2
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
289074
Reizer
Exopolyphosphate phosphatase a ...
Escherichia coli
Trends Biochem. Sci.
18
247-248
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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2
-
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1
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-
-
2
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
289072
Hara
Guanosine 5'-triphosphate, 3'- ...
Escherichia coli, Escherichia coli JF1599
J. Biol. Chem.
258
1678-1683
1983
-
-
-
-
-
-
-
2
-
3
1
2
-
3
-
-
1
-
-
-
2
1
11
-
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-
-
1
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2
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3
1
2
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1
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2
1
11
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1
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