Protein Variants | Comment | Organism |
---|---|---|
E343Q | site-directed mutagenesis, the mutant is inactive, dTTP hydrolysis and DNA unwinding activities are abolished, but the mutant shows increased single-stranded DNA binding affinity compared to the wild-type enzyme, overview | Escherichia phage T7 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia phage T7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + H2O | Escherichia phage T7 | mechanisms of DNA-dependent and-independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed. In the presence of dTTP, gene 4 protein monomers assemble as a ring that binds single-stranded DNA and couples the hydrolysis of dTTP to unidirectional translocation and the unwinding of duplex DNA, overview | dTDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage T7 | - |
the enzyme is encoded on gene 4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + H2O | mechanisms of DNA-dependent and-independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed. In the presence of dTTP, gene 4 protein monomers assemble as a ring that binds single-stranded DNA and couples the hydrolysis of dTTP to unidirectional translocation and the unwinding of duplex DNA, overview | Escherichia phage T7 | dTDP + phosphate | - |
? | |
dTTP + H2O | the lysines on loop II are essential for ssDNA binding | Escherichia phage T7 | dTDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | all subunits are catalytic | Escherichia phage T7 |
Synonyms | Comment | Organism |
---|---|---|
dTTPase | - |
Escherichia phage T7 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
DNA binding and dTTPase assays at | Escherichia phage T7 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
DNA binding and dTTPase assays at | Escherichia phage T7 |