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Literature summary for 3.6.1.29 extracted from
- Fhit proteins can also recognize substrates other than dinucleoside polyphosphates (2008), FEBS Lett., 582, 3152-3158.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene At5g58240, DNA and amino acid sequence determination and analysis | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 5'-O-(gamma-fluoromonophosphate) | - |
Arabidopsis thaliana |  |
| adenosine 5'-O-(gamma-fluorotriphosphate) | - |
Arabidopsis thaliana | |
| adenosine 5'-phosphosulfate | substrate inhibition | Arabidopsis thaliana | |
| adenosine 5'-phosphosulfate | substrate inhibition | Homo sapiens | |
| ADP | - |
Arabidopsis thaliana | |
| AMP | product inhibition | Arabidopsis thaliana | |
| AMP | product inhibition | Homo sapiens | |
| ATP | - |
Arabidopsis thaliana | |
| additional information | poor inhibition by fluoroadenylates, ATP, and ADP | Homo sapiens | |
| P1,P3-bis(5'-adenosyl) triphosphate | substrate inhibition | Arabidopsis thaliana | |
| P1,P3-bis(5'-adenosyl) triphosphate | substrate inhibition | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
adenosine 5'-phosphoramidate | pH 6.8, 30°C | Homo sapiens | |
| 0.003 | - |
5'-O-[amino(hydroxy)phosphoryl]adenosine | pH 6.8, 30°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Arabidopsis thaliana | |
| additional information | no requirement for Mg2+ | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine 5'-phosphoramidate + H2O | Homo sapiens | - |
AMP + NH3 | - |
? | |
| adenosine 5'-phosphoramidate + H2O | Arabidopsis thaliana | no requirement for Mg2+ | AMP + NH3 | - |
? | |
| adenosine 5'-phosphosulfate + H2O | Arabidopsis thaliana | no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase | AMP + sulfate | - |
? | |
| adenosine 5'-phosphosulfate + H2O | Homo sapiens | reaction of EC 3.6.2.1, adenylylsulfatase | AMP + sulfate | - |
? | |
| adenylylfluoride + H2O | Arabidopsis thaliana | - |
AMP + fluoride | - |
? | |
| gamma-fluoro-ATP + H2O | Arabidopsis thaliana | - |
AMP + fluorodiphosphoric acid | - |
? | |
| additional information | Arabidopsis thaliana | Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview | ? | - |
? | |
| additional information | Homo sapiens | Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview | ? | - |
? | |
| P1,P3-bis(5'-adenosyl) triphosphate + H2O | Homo sapiens | - |
ADP + AMP | - |
? | |
| P1,P3-bis(5'-adenosyl) triphosphate + H2O | Arabidopsis thaliana | requirement for Mg2+ | ADP + AMP | - |
? | |
| P1-bis(5'-adenosyl) monophosphate + H2O | Arabidopsis thaliana | requirement for Mg2+ | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9LVM4 | gene FHIT | - |
| Homo sapiens | - |
gene FHIT | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative velocities of hydrolysis with different substrates, overview | Homo sapiens |
| additional information | - |
relative velocities of hydrolysis with different substrates, overview | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine 5'-phosphoramidate + H2O | - |
Homo sapiens | AMP + NH3 | - |
? | |
| adenosine 5'-phosphoramidate + H2O | preferred substrate | Homo sapiens | AMP + NH3 | - |
? | |
| adenosine 5'-phosphoramidate + H2O | preferred substrate | Arabidopsis thaliana | AMP + NH3 | - |
? | |
| adenosine 5'-phosphoramidate + H2O | no requirement for Mg2+ | Arabidopsis thaliana | AMP + NH3 | - |
? | |
| adenosine 5'-phosphosulfate + H2O | no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase | Arabidopsis thaliana | AMP + sulfate | - |
? | |
| adenosine 5'-phosphosulfate + H2O | reaction of EC 3.6.2.1, adenylylsulfatase | Homo sapiens | AMP + sulfate | - |
? | |
| adenosine 5'-phosphosulfate + H2O | reaction of EC 3.6.2.1, adenylylsulfatase | Arabidopsis thaliana | AMP + sulfate | - |
? | |
| adenylylfluoride + H2O | - |
Arabidopsis thaliana | AMP + fluoride | - |
? | |
| gamma-fluoro-ATP + H2O | - |
Arabidopsis thaliana | AMP + fluorodiphosphoric acid | - |
? | |
| inosine 5'-phosphoramidate + H2O | - |
Homo sapiens | IMP + NH3 | - |
? | |
| inosine 5'-phosphoramidate + H2O | - |
Arabidopsis thaliana | IMP + NH3 | - |
? | |
| additional information | Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview | Homo sapiens | ? | - |
? | |
| additional information | the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP | Homo sapiens | ? | - |
? | |
| additional information | the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP | Arabidopsis thaliana | ? | - |
? | |
| P1,P3-bis(5'-adenosyl) triphosphate + H2O | - |
Homo sapiens | ADP + AMP | - |
? | |
| P1,P3-bis(5'-adenosyl) triphosphate + H2O | - |
Arabidopsis thaliana | ADP + AMP | - |
? | |
| P1,P3-bis(5'-adenosyl) triphosphate + H2O | requirement for Mg2+ | Arabidopsis thaliana | ADP + AMP | - |
? | |
| P1-bis(5'-adenosyl) monophosphate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| P1-bis(5'-adenosyl) monophosphate + H2O | requirement for Mg2+ | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dinucleoside triphosphatase | - |
Homo sapiens |
| dinucleoside triphosphatase | - |
Arabidopsis thaliana |
| Fhit protein | - |
Homo sapiens |
| Fhit protein | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.26 | - |
adenosine 5'-phosphoramidate | pH 6.8, 30°C | Homo sapiens | |
| 1.26 | - |
5'-O-[amino(hydroxy)phosphoryl]adenosine | pH 6.8, 30°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Homo sapiens |
| 6.8 | - |
assay at | Arabidopsis thaliana |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
P1,P3-bis(5'-adenosyl) triphosphate | pH 6.8, 30°C, in presence of Mg2+ | Arabidopsis thaliana | |
| 0.0071 | - |
adenosine 5'-phosphosulfate | pH 6.8, 30°C | Arabidopsis thaliana | |
| 0.0136 | - |
adenosine 5'-phosphosulfate | pH 6.8, 30°C | Homo sapiens | |
| 0.018 | - |
adenosine 5'-O-(gamma-fluoromonophosphate) | pH 6.8, 30°C | Arabidopsis thaliana | |
| 0.018 | - |
P1,P3-bis(5'-adenosyl) triphosphate | pH 6.8, 30°C, in presence of Mg2+ | Homo sapiens | |
| 0.11 | - |
AMP | pH 6.8, 30°C | Homo sapiens | |
| 0.113 | - |
AMP | pH 6.8, 30°C | Arabidopsis thaliana | |
| 0.14 | - |
ADP | pH 6.8, 30°C | Arabidopsis thaliana | |
| 0.145 | - |
ATP | pH 6.8, 30°C | Arabidopsis thaliana | |
| 0.235 | - |
adenosine 5'-O-(gamma-fluoromonophosphate) | pH 6.8, 30°C, in presence of Mg2+ | Arabidopsis thaliana | |
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