Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.29 extracted from

  • Pawelczyk, T.; Kowara, R.; Golebiowski, F.; Matecki, A.
    Expression in Escherichia coli and simple purification of human Fhit protein (2000), Protein Expr. Purif., 18, 320-326.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20500
-
alpha2, 2 * 20500, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
P1-P3-bis(5'-adenosyl) triphosphate + H2O Homo sapiens
-
ADP + AMP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P49789
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 50 mM HEPES-NaOH buffer, pH 7.0, 100 mM NaCl, 25% glycerol, at least 18 months Homo sapiens
4°C, 50 mM HEPES-NaOH buffer, pH 7.0, 100 mM NaCl, 3 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
Homo sapiens ADP + AMP
-
?

Subunits

Subunits Comment Organism
homodimer alpha2, 2 * 20500, SDS-PAGE Homo sapiens