Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Mus musculus |
Crystallization (Comment) | Organism |
---|---|
complex with tripolyphosphate, to 2.3 A resolution and docking solution of substrate thiamine triphosphate. The thiazole ring of the thiamine forms a stacking interaction with Trp53, and the aminopyrimidine ring lies in a pocket defined by His76, Pro191 and Ile195. The triphosphate moiety occupies a similar position as in the enzyme-tripolyphosphate complex, with interactions involving the side chains of Lys11, Arg55, Arg57, Lys65, Arg125 and Lys193 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D147A | about 30% of wild-type catalytic efficiency | Mus musculus |
D37A | about 0.5% of wild-type catalytic efficiency | Mus musculus |
E81A | about 30% of wild-type catalytic efficiency | Mus musculus |
K11A | about 15% of wild-type catalytic efficiency | Mus musculus |
K193A | about 60% of wild-type catalytic efficiency | Mus musculus |
K65A | more than 1000fold decrease in kcat value | Mus musculus |
W53A | 2.5fold increase in catalytic efficiency | Mus musculus |
Y39A | about 30% of wild-type catalytic efficiency | Mus musculus |
Y79A | about 5% of wild-type catalytic efficiency | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
thiamine triphosphate | wild-type, pH 8.5, 37°C | Mus musculus | |
0.009 | - |
thiamine triphosphate | mutant K65A, pH 8.5, 37°C | Mus musculus | |
0.012 | - |
thiamine triphosphate | mutant K11A, pH 8.5, 37°C | Mus musculus | |
0.013 | - |
thiamine triphosphate | mutant E81A, pH 8.5, 37°C | Mus musculus | |
0.042 | - |
thiamine triphosphate | mutant Y79A, pH 8.5, 37°C | Mus musculus | |
0.2 | - |
thiamine triphosphate | mutant D37A, pH 8.5, 37°C | Mus musculus | |
0.55 | - |
thiamine triphosphate | mutant W53A, pH 8.5, 37°C | Mus musculus | |
0.62 | - |
thiamine triphosphate | mutant Y39A, pH 8.5, 37°C | Mus musculus | |
0.74 | - |
thiamine triphosphate | mutant K193A, pH 8.5, 37°C | Mus musculus | |
2.8 | - |
thiamine triphosphate | mutant D147A, pH 8.5, 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for catalytic activity, not required for substrate binding | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BU02 | - |
- |
Mus musculus | Q8JZL3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine triphosphate + H2O | - |
Mus musculus | thiamine diphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Thtpa | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
thiamine triphosphate | mutant K65A, pH 8.5, 37°C | Mus musculus | |
0.64 | - |
thiamine triphosphate | mutant Y39A, pH 8.5, 37°C | Mus musculus | |
1.5 | - |
thiamine triphosphate | mutant K193A, pH 8.5, 37°C | Mus musculus | |
2.6 | - |
thiamine triphosphate | mutant D147A, pH 8.5, 37°C | Mus musculus | |
2.8 | - |
thiamine triphosphate | mutant D37A, pH 8.5, 37°C | Mus musculus | |
4.4 | - |
thiamine triphosphate | mutant W53A, pH 8.5, 37°C | Mus musculus | |
6 | - |
thiamine triphosphate | mutant K11A, pH 8.5, 37°C | Mus musculus | |
6 | - |
thiamine triphosphate | mutant Y79A, pH 8.5, 37°C | Mus musculus | |
14.1 | - |
thiamine triphosphate | mutant E81A, pH 8.5, 37°C | Mus musculus | |
23 | - |
thiamine triphosphate | wild-type, pH 8.5, 37°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
thiamine triphosphate | mutant D37A, pH 8.5, 37°C | Mus musculus | |
140 | - |
thiamine triphosphate | mutant Y79A, pH 8.5, 37°C | Mus musculus | |
500 | - |
thiamine triphosphate | mutant K11A, pH 8.5, 37°C | Mus musculus | |
1000 | - |
thiamine triphosphate | mutant D147A, pH 8.5, 37°C | Mus musculus | |
1000 | - |
thiamine triphosphate | mutant E81A, pH 8.5, 37°C | Mus musculus | |
1000 | - |
thiamine triphosphate | mutant Y39A, pH 8.5, 37°C | Mus musculus | |
2000 | - |
thiamine triphosphate | mutant K193A, pH 8.5, 37°C | Mus musculus | |
2000 | - |
thiamine triphosphate | mutant K65A, pH 8.5, 37°C | Mus musculus | |
3000 | - |
thiamine triphosphate | wild-type, pH 8.5, 37°C | Mus musculus | |
8000 | - |
thiamine triphosphate | mutant W53A, pH 8.5, 37°C | Mus musculus |