BRENDA - Enzyme Database show
show all sequences of 3.6.1.28

The iron-regulated metastasis suppressor, Ndrg-1: identification of novel molecular targets

Kovacevic, Z.; Fu, D.; Richardson, D.R.; Biochim. Biophys. Acta 1783, 1981-1992 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Homo sapiens
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Rattus norvegicus
Cloned(Commentary)
Commentary
Organism
expression analysis
Homo sapiens
expression analysis
Rattus norvegicus
Engineering
Amino acid exchange
Commentary
Organism
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Homo sapiens
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
?
-
-
-
additional information
Rattus norvegicus
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Rattus norvegicus
Q8CGV7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
AT6.1 cell
metastatic prostate cancer cells
Rattus norvegicus
-
DLD-1 cell
non-metastatic human colorectal adenocarcinoma cells
Homo sapiens
-
NCI-H1299 cell
metastatic lung cancer cells
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
696404
Homo sapiens
?
-
-
-
-
additional information
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
696404
Rattus norvegicus
?
-
-
-
-
thiamine triphosphate + H2O
-
696404
Homo sapiens
thiamine diphosphate + phosphate
-
-
-
?
thiamine triphosphate + H2O
-
696404
Rattus norvegicus
thiamine diphosphate + phosphate
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Homo sapiens
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression analysis
Homo sapiens
expression analysis
Rattus norvegicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Homo sapiens
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
?
-
-
-
additional information
Rattus norvegicus
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
AT6.1 cell
metastatic prostate cancer cells
Rattus norvegicus
-
DLD-1 cell
non-metastatic human colorectal adenocarcinoma cells
Homo sapiens
-
NCI-H1299 cell
metastatic lung cancer cells
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
696404
Homo sapiens
?
-
-
-
-
additional information
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
696404
Rattus norvegicus
?
-
-
-
-
thiamine triphosphate + H2O
-
696404
Homo sapiens
thiamine diphosphate + phosphate
-
-
-
?
thiamine triphosphate + H2O
-
696404
Rattus norvegicus
thiamine diphosphate + phosphate
-
-
-
?
Other publictions for EC 3.6.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751047
Martinez
Structural determinants for s ...
Mus musculus
J. Biol. Chem.
290
23348-23360
2015
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1
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1
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1
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3
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1
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1
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1
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1
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1
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-
733393
Delvaux
Structural determinants of spe ...
Homo sapiens, Mus musculus
Biochim. Biophys. Acta
1830
4513-4523
2013
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-
2
1
9
-
-
10
-
1
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2
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1
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9
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2
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1
9
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10
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1
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1
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9
-
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7
7
687693
Song
Structural basis for the catal ...
Mus musculus
J. Biol. Chem.
283
10939-10948
2008
-
-
-
-
-
-
1
1
1
1
1
1
-
2
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1
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2
1
1
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1
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1
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1
1
1
1
1
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-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
696404
Kovacevic
The iron-regulated metastasis ...
Homo sapiens, Rattus norvegicus
Biochim. Biophys. Acta
1783
1981-1992
2008
2
-
2
-
2
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2
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2
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3
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4
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2
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2
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2
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2
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3
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4
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-
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-
667797
Szyniarowski Piot
Pig tissues express a catalyti ...
Homo sapiens, Sus scrofa
Biochim. Biophys. Acta
1725
93-102
2005
-
-
2
-
4
-
2
3
-
5
2
-
-
6
-
-
2
-
-
1
16
-
2
-
-
-
1
-
5
-
-
-
2
-
1
-
-
2
-
-
4
-
1
2
2
3
-
5
2
-
-
-
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2
-
1
16
-
2
-
-
-
1
-
5
-
-
-
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-
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-
655738
Lakaye
Human recombinant thiamine tri ...
Homo sapiens
Int. J. Biochem. Cell Biol.
36
1348-1364
2004
-
-
1
-
-
-
6
2
1
2
-
-
-
3
-
-
1
-
-
-
1
-
2
-
-
-
2
2
1
1
-
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2
-
1
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-
1
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1
7
2
2
1
2
-
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1
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-
1
-
2
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-
2
2
1
1
-
-
-
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-
-
-
-
656873
Czerniecki
Neuronal localization of the 2 ...
Rattus norvegicus
Neuroscience
125
833-840
2004
-
-
-
-
-
-
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-
1
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1
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4
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2
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668963
Lakaye
Expression of 25 kDa thiamine ...
Mus musculus, Rattus norvegicus
Int. J. Biochem. Cell Biol.
36
2032-2041
2004
-
-
-
-
-
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1
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5
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16
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2
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1
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16
-
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2
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-
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-
655198
Makarchikov
Thiamine triphosphate and thia ...
Bos taurus, Gallus gallus, Homo sapiens, quail, Rattus norvegicus, Sus scrofa
Cell. Mol. Life Sci.
60
1477-1488
2003
-
-
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15
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17
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12
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25
22
-
6
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-
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15
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17
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25
22
-
6
-
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-
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-
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-
-
-
-
-
-
656042
Lakaye
Molecular characterization of ...
Bos taurus, Homo sapiens
J. Biol. Chem.
277
13771-13777
2002
-
-
1
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-
-
4
2
1
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6
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7
-
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2
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9
2
-
4
2
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2
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1
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4
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2
1
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6
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2
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9
2
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4
2
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-
-
2
-
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-
-
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-
-
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-
210023
Makarchikov
Thiamine triphosphatase activi ...
Bos taurus
Biochem. Mol. Biol. Int.
46
115-123
1998
-
-
-
-
-
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2
-
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1
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2
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1
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2
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1
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2
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1
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1
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2
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1
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-
210021
Bettendorff
Thiamine, thiamine phosphates, ...
Homo sapiens
J. Neurochem.
66
250-258
1996
-
-
-
-
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-
1
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1
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1
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1
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1
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1
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1
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-
1
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-
-
-
1
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-
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-
-
-
-
-
-
210020
Makarchikov
Purification and characterizat ...
Bos taurus
Biochim. Biophys. Acta
1117
326-332
1992
-
-
-
-
-
1
-
1
1
6
2
-
-
2
-
-
1
-
-
2
1
2
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
6
2
-
-
-
-
1
-
2
1
2
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
210012
Matsuda
Membrane-associated thiamine t ...
Rattus norvegicus
Int. J. Biochem.
23
1111-1114
1991
-
-
-
-
-
-
9
-
1
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-
-
2
-
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-
2
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3
-
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-
2
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9
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1
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2
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3
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2
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210022
Bettendorff
Solubilization and thiamine tr ...
Electrophorus electricus
Biochim. Biophys. Acta
1073
69-76
1991
4
-
-
-
-
-
3
-
1
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2
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2
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1
1
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1
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4
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3
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1
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2
-
1
1
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1
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210024
Bettendorff
Application of high-performanc ...
Electrophorus electricus
J. Chromatogr.
566
397-408
1991
-
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-
-
-
-
-
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1
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1
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3
-
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1
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1
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1
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1
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2
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210019
Nishimune
Hydrolysis and synthesis of th ...
Aneurinibacillus aneurinilyticus, Escherichia coli, Escherichia coli W 1485
J. Nutr. Sci. Vitaminol.
33
113-127
1987
-
-
-
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3
2
2
1
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3
-
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3
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2
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2
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1
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3
1
2
2
1
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3
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2
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2
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210018
Ogawa
Recent findings on ultracytoch ...
Rattus norvegicus
Ann. N. Y. Acad. Sci.
378
188-214
1982
-
-
-
-
-
-
-
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4
-
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1
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1
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3
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2
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4
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1
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3
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2
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210017
Penttinen
The relation of the soluble th ...
Rattus norvegicus
Med. Biol.
59
177-184
1981
-
-
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2
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2
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7
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2
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7
-
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-
-
-
210013
Barchi
Membrane-associated thiamin tr ...
Rattus norvegicus
J. Biol. Chem.
251
193-197
1976
-
-
-
-
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1
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1
3
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1
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1
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1
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1
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1
3
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1
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1
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-
210014
Barchi
Membrane thiamine triphosphata ...
Rattus norvegicus
J. Neurochem.
26
715-720
1976
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4
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2
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2
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2
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1
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4
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2
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2
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1
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210011
Iwata
Properties of thiamine di- and ...
Rattus norvegicus
J. Neurochem.
24
1209-1213
1975
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4
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2
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1
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1
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1
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4
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1
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1
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210009
Iwata
Role of thiamine metabolism in ...
Rattus norvegicus
Jpn. J. Pharmacol.
24
825-829
1974
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2
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1
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2
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3
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1
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2
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1
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3
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1
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210010
Iwata
Role of thiamine metabolism in ...
Rattus norvegicus
Jpn. J. Pharmacol.
24
817-823
1974
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1
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3
2
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2
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3
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4
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3
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1
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3
2
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3
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4
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3
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210015
Iwata
-
Some properties of the enzyme ...
Rattus norvegicus
Thiamine (Proc. Pap. U. S. -Jpn. Semin, 2. Meeting)
2. Meeting
213-221
1974
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4
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2
2
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1
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2
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1
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4
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2
2
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2
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1
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210016
Barchi
-
Thiamine triphosphatases in br ...
Rattus norvegicus
Thiamine (Proc. Pap. U. S. -Jpn. Semin, 2. Meeting)
195-212
1974
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5
2
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3
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1
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1
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1
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1
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1
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2
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5
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2
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3
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1
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1
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1
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1
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2
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210006
Barchi
A membrane-associated thiamine ...
Rattus norvegicus
J. Biol. Chem.
247
7668-7673
1972
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3
3
1
8
1
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2
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1
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8
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1
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1
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2
2
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3
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3
1
8
1
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1
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8
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1
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1
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2
2
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210007
Hashitani
The partial purification of th ...
Rattus norvegicus
J. Biol. Chem.
247
2117-2119
1972
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4
1
1
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2
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6
1
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4
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4
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1
1
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6
1
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4
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210008
Barchi
Thiamine triphosphatase in the ...
Rattus norvegicus
Biochim. Biophys. Acta
255
402-405
1972
1
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