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Literature summary for 3.6.1.28 extracted from
- Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues (2002), J. Biol. Chem., 277, 13771-13777.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli as a glutathione S-transferase fusion protein | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis-2-nitrobenzoic acid | - |
Bos taurus |  |
| 5,5'-dithiobis-2-nitrobenzoic acid | - |
Homo sapiens | |
| PCMB | - |
Bos taurus | |
| PCMB | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
thiamine triphosphate | - |
Bos taurus | |
| 0.126 | - |
thiamine triphosphate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23892 | - |
1 * 23892, mass spectrometry | Homo sapiens |
| 23892 | - |
1 * 23892, mass spectrometry | Bos taurus |
| 25000 | - |
gel filtration | Homo sapiens |
| 25000 | - |
gel filtration | Bos taurus |
| 27000 | - |
1 * 27000, SDS-PAGE | Homo sapiens |
| 27000 | - |
1 * 27000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | Q8MKF1 | calf | - |
| Homo sapiens | Q9BU02 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bladder | - |
Homo sapiens | - |
| brain | - |
Bos taurus | - |
| brain | low activity | Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| prostate | high activity | Homo sapiens | - |
| testis | high activity | Homo sapiens | - |
| thyroid gland | - |
Homo sapiens | - |
| uterus | high activity | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 103 | - |
- |
Homo sapiens |
| 103 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine triphosphate + H2O | - |
Homo sapiens | thiamine diphosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | - |
Bos taurus | thiamine diphosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | absolute specificity | Homo sapiens | thiamine diphosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | absolute specificity | Bos taurus | thiamine diphosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, SDS-PAGE | Homo sapiens |
| monomer | 1 * 27000, SDS-PAGE | Bos taurus |
| monomer | 1 * 23892, mass spectrometry | Homo sapiens |
| monomer | 1 * 23892, mass spectrometry | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ThTPase | - |
Homo sapiens |
| ThTPase | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 240 | - |
thiamine triphosphate | - |
Homo sapiens | |
| 240 | - |
thiamine triphosphate | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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