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Literature summary for 3.6.1.28 extracted from
- Purification and characterization of thiamine triphosphatase from bovine brain (1992), Biochim. Biophys. Acta, 1117, 326-332.
General Stability
| General Stability | Organism |
|---|---|
| not inactivated by freezing and thawing | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
thiamine triphosphate | - |
Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 7.3% of the activation with Mg2+ | Bos taurus | |
| Ca2+ | 12.7% of the activation with Mg2+ | Bos taurus | |
| Mg2+ | absolute requirement for divalent cations, Mg2+ is most effective | Bos taurus | |
| Mn2+ | 30% of the activation with Mg2+ | Bos taurus | |
| Ni2+ | 30% of the activation with Mg2+ | Bos taurus | |
| Zn2+ | 30% of the activation with Mg2+ | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33350 | - |
gel filtration | Bos taurus |
| 33900 | - |
1 * 33900, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 648 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.05 M Tris-HCl buffer, stable for several months | Bos taurus |
| 4°C, stable for about 2 weeks | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | no activity | Bos taurus | ADP + phosphate | - |
? | |
| CTP + H2O | no activity | Bos taurus | CDP + phosphate | - |
? | |
| GTP + H2O | no activity | Bos taurus | GDP + phosphate | - |
? | |
| ITP + H2O | no activity | Bos taurus | IDP + phosphate | - |
? | |
| thiamine diphosphate + H2O | no activity | Bos taurus | thiamine monophosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | - |
Bos taurus | thiamine diphosphate + phosphate | - |
? | |
| UTP + H2O | no activity | Bos taurus | UDP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33900, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5: about 30% of maximal activity, pH 9.5: about 50% of maximal activity | Bos taurus |
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