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Literature summary for 3.6.1.28 extracted from
- Hydrolysis and synthesis of thiamin triphosphate in bacteria (1987), J. Nutr. Sci. Vitaminol., 33, 113-127.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-3-(2-hydroxyethyl)-2-methylpyridinium | soluble enzyme: 50% inhibition at 2 mM. Membrane-bound enzyme: no inhibition up to 5 mM | Escherichia coli |  |
| ATP | soluble enzyme: no inhibition up to 2 mM, membrane-bound enzyme: 50% inhibition at 5 mM | Escherichia coli | |
| Ca2+ | soluble enzyme: 50% inhibition at 5 mM, membrane-bound enzyme: 20% inhibition at 5 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
thiamine triphosphate | membrane-bound enzyme | Escherichia coli | |
| 0.9 | - |
thiamine triphosphate | soluble enzyme | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated | Escherichia coli | 16020 | - |
| soluble | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | soluble enzyme requires Mg2+, enzyme from membrane is Mg2+-independent | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aneurinibacillus aneurinilyticus | - |
- |
- |
| Escherichia coli | - |
W 1485 | - |
| Escherichia coli W 1485 | - |
W 1485 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine triphosphate + H2O | - |
Escherichia coli | thiamine diphosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | - |
Aneurinibacillus aneurinilyticus | thiamine diphosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | - |
Escherichia coli W 1485 | thiamine diphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
membrane-bound enzyme | Escherichia coli |
| 44 | - |
soluble enzyme | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
membrane-bound enzyme | Escherichia coli |
| 7 | - |
soluble enzyme | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
1-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-3-(2-hydroxyethyl)-2-methylpyridinium | soluble enzyme | Escherichia coli | |
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