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Literature summary for 3.6.1.27 extracted from

  • Chang, H.Y.; Chou, C.C.; Wu, M.L.; Wang, A.H.J.
    Expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus (2017), Protein Expr. Purif., 133, 121-131 .
    View publication on PubMed
Search for more literature for 3.6.1.27

Activating Compound

Activating Compound Comment Organism Structure
n-dodecyl beta-D-maltoside the enzyme presents high activity, greater than 90%, with 0.02-1% (v/v) n-dodecyl beta-D-maltoside Vibrio vulnificus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 (DE3) cells Vibrio vulnificus

Protein Variants

Protein Variants Comment Organism
E17A inactive Vibrio vulnificus
G163A inactive Vibrio vulnificus
G163R the mutation leads to no protein expression at all Vibrio vulnificus
G168A the mutation leads to no protein expression at all Vibrio vulnificus
H26A inactive Vibrio vulnificus
Q13A inactive Vibrio vulnificus
R166A inactive Vibrio vulnificus
S165A inactive Vibrio vulnificus
S167A the mutant shows 34% of wild type activity Vibrio vulnificus
S22A the mutant shows less than 1.3% of wild type activity Vibrio vulnificus
S23A the mutant shows 65% of wild type activity Vibrio vulnificus

Inhibitors

Inhibitors Comment Organism Structure
bacitracin
-
 Vibrio vulnificus
Ca2+ about less than 10% residual activity at 10 mM Vibrio vulnificus
Cu2+ complete inhibition at 10 mM Vibrio vulnificus
EDTA complete inhibition at 0.001 mM Vibrio vulnificus
Mn2+ about 90% residual activity at 10 mM Vibrio vulnificus
Zn2+ about less than 10% residual activity at 10 mM Vibrio vulnificus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00846
-
 farnesyl diphosphate mutant enzyme S167A, at pH 7.5 and 37°C Vibrio vulnificus
0.0106
-
 farnesyl diphosphate wild type enzyme, at pH 7.5 and 37°C Vibrio vulnificus
0.01127
-
 farnesyl diphosphate mutant enzyme S23A, at pH 7.5 and 37°C Vibrio vulnificus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Vibrio vulnificus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 130% activity at 10 mM Vibrio vulnificus
additional information not affected by Mg2+ Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
undecaprenyl diphosphate + H2O Vibrio vulnificus
-
 undecaprenyl phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio vulnificus A0A087INM7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + H2O
-
 Vibrio vulnificus farnesyl phosphate + phosphate
-
 ?
undecaprenyl diphosphate + H2O
-
 Vibrio vulnificus undecaprenyl phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Vibrio vulnificus

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate phosphatase
-
 Vibrio vulnificus
UppP
-
 Vibrio vulnificus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 farnesyl diphosphate mutant enzyme S167A, at pH 7.5 and 37°C Vibrio vulnificus
1.67
-
 farnesyl diphosphate mutant enzyme S23A, at pH 7.5 and 37°C Vibrio vulnificus
2.3
-
 farnesyl diphosphate wild type enzyme, at pH 7.5 and 37°C Vibrio vulnificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Vibrio vulnificus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 the mutant shows more than 50% activity between pH 6.0 and 9.0 Vibrio vulnificus