Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lauryldimethylamine oxide | the enzyme shows optimal activity at 0.1% (v/v) lauryldimethylamine oxide (4.3 mM) | Escherichia coli | |
n-dodecyl-beta-D-maltopyranoside | the enzyme activity appears optimal at low n-dodecyl-beta-D-maltopyranoside concentrations around 0.050-15% (v/v) (1-3 mM) | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E21A | the mutant shows 1.4% of wild type activity | Escherichia coli |
H30A | the mutant shows 6.2% of wild type activity | Escherichia coli |
Q164A | the mutant shows 24.3% of wild type activity | Escherichia coli |
R174A | the mutant shows 0.13% of wild type activity | Escherichia coli |
R189A | the mutant shows 12.1% of wild type activity | Escherichia coli |
S173A | the mutant shows 2.2% of wild type activity | Escherichia coli |
S175A | the mutant shows 41% of wild type activity | Escherichia coli |
S196A | the mutant shows 36.8% of wild type activity | Escherichia coli |
S26A | the mutant shows 15% of wild type activity | Escherichia coli |
S27A | the mutant shows 0.013% of wild type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 95% inhibition at 0.05 mM, 98% inhibition at 1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
undecaprenyl diphosphate | at pH 7.5 and 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme activity lost after treatment with 0.05 mM EDTA is not only fully recovered following addition of Ca2+ (1 mM) but also greatly enhanced by an about 9fold factor | Escherichia coli | |
Mg2+ | the enzyme activity lost after treatment with 0.05 mM EDTA is fully recovered following addition of Mg2+ (1 mM) | Escherichia coli | |
Mn2+ | the enzyme activity lost after treatment with 0.05 mM EDTA is partially recovered following addition of Mn2+ (1 mM) | Escherichia coli | |
additional information | no activity with Co2+, Fe2+, Zn2+, and Cu2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
undecaprenyl diphosphate + H2O | Escherichia coli | - |
undecaprenyl phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex S200 gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.5 | - |
with diacylglycerol diphosphate as substrate, at pH 7.5 and 37°C | Escherichia coli |
10.3 | - |
with farnesyl diphosphate as substrate, at pH 7.5 and 37°C | Escherichia coli |
11.3 | - |
with undecaprenyl diphosphate as substrate, at pH 7.5 and 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacylglycerol diphosphate + H2O | relatively good substrate | Escherichia coli | diacylglycerol phosphate + phosphate | - |
? | |
farnesyl diphosphate + H2O | - |
Escherichia coli | farnesyl phosphate + phosphate | - |
? | |
additional information | no activity with isopentenyl diphosphate, phosphatidylglycerol phosphate and phosphatidic acid | Escherichia coli | ? | - |
- |
|
undecaprenyl diphosphate + H2O | - |
Escherichia coli | undecaprenyl phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
bacA | - |
Escherichia coli |
C55-PP phosphatase | - |
Escherichia coli |
undecaprenyl-pyrophosphate phosphatase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | the enzyme has optimal activity at 65-70°C which is about 16 fold higher than at 37°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
73.5 | - |
melting temperature | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27 | - |
undecaprenyl diphosphate | at pH 7.5 and 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | a plateau of maximal activity is observed between 5.0 and 7.5. The enzyme does not show significant activity at acid pH values below 5.0 | Escherichia coli |