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Literature summary for 3.6.1.27 extracted from
- Membrane topology and biochemical characterization of the Escherichia coli BacA undecaprenyl-pyrophosphate phosphatase (2015), PLoS ONE, 10, e0142870 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lauryldimethylamine oxide | the enzyme shows optimal activity at 0.1% (v/v) lauryldimethylamine oxide (4.3 mM) | Escherichia coli |  |
| n-dodecyl-beta-D-maltopyranoside | the enzyme activity appears optimal at low n-dodecyl-beta-D-maltopyranoside concentrations around 0.050-15% (v/v) (1-3 mM) | Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C43(DE3) cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E21A | the mutant shows 1.4% of wild type activity | Escherichia coli |
| H30A | the mutant shows 6.2% of wild type activity | Escherichia coli |
| Q164A | the mutant shows 24.3% of wild type activity | Escherichia coli |
| R174A | the mutant shows 0.13% of wild type activity | Escherichia coli |
| R189A | the mutant shows 12.1% of wild type activity | Escherichia coli |
| S173A | the mutant shows 2.2% of wild type activity | Escherichia coli |
| S175A | the mutant shows 41% of wild type activity | Escherichia coli |
| S196A | the mutant shows 36.8% of wild type activity | Escherichia coli |
| S26A | the mutant shows 15% of wild type activity | Escherichia coli |
| S27A | the mutant shows 0.013% of wild type activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 95% inhibition at 0.05 mM, 98% inhibition at 1 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.68 | - |
undecaprenyl diphosphate | at pH 7.5 and 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme activity lost after treatment with 0.05 mM EDTA is not only fully recovered following addition of Ca2+ (1 mM) but also greatly enhanced by an about 9fold factor | Escherichia coli | |
| Mg2+ | the enzyme activity lost after treatment with 0.05 mM EDTA is fully recovered following addition of Mg2+ (1 mM) | Escherichia coli | |
| Mn2+ | the enzyme activity lost after treatment with 0.05 mM EDTA is partially recovered following addition of Mn2+ (1 mM) | Escherichia coli | |
| additional information | no activity with Co2+, Fe2+, Zn2+, and Cu2+ | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| undecaprenyl diphosphate + H2O | Escherichia coli | - |
undecaprenyl phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex S200 gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
with diacylglycerol diphosphate as substrate, at pH 7.5 and 37°C | Escherichia coli |
| 10.3 | - |
with farnesyl diphosphate as substrate, at pH 7.5 and 37°C | Escherichia coli |
| 11.3 | - |
with undecaprenyl diphosphate as substrate, at pH 7.5 and 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylglycerol diphosphate + H2O | relatively good substrate | Escherichia coli | diacylglycerol phosphate + phosphate | - |
? | |
| farnesyl diphosphate + H2O | - |
Escherichia coli | farnesyl phosphate + phosphate | - |
? | |
| additional information | no activity with isopentenyl diphosphate, phosphatidylglycerol phosphate and phosphatidic acid | Escherichia coli | ? | - |
- |
|
| undecaprenyl diphosphate + H2O | - |
Escherichia coli | undecaprenyl phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacA | - |
Escherichia coli |
| C55-PP phosphatase | - |
Escherichia coli |
| undecaprenyl-pyrophosphate phosphatase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 70 | the enzyme has optimal activity at 65-70°C which is about 16 fold higher than at 37°C | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 73.5 | - |
melting temperature | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27 | - |
undecaprenyl diphosphate | at pH 7.5 and 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | a plateau of maximal activity is observed between 5.0 and 7.5. The enzyme does not show significant activity at acid pH values below 5.0 | Escherichia coli |
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