BRENDA - Enzyme Database show
show all sequences of 3.6.1.27

Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus

Goldman, R.; Strominger, J.L.; J. Biol. Chem. 247, 5116-5122 (1972)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethanol
slight activation at concentration of 4 M
Micrococcus luteus
methanol
slight activation at concentration of 4 M
Micrococcus luteus
additional information
significant rise in activity at high ionic strength
Micrococcus luteus
n-Propanol
activating at concentrations below 1.5 M
Micrococcus luteus
Triton X-100
-
Micrococcus luteus
Triton X-114
-
Micrococcus luteus
General Stability
General Stability
Organism
ascorbic acid stabilizes
Micrococcus luteus
Inhibitors
Inhibitors
Commentary
Organism
Structure
DTNB
slight inhibition
Micrococcus luteus
n-butanol
-
Micrococcus luteus
n-Propanol
inhibitory at concentrations above 1.5 M
Micrococcus luteus
p-chloromercuriphenylsulfonic acid
-
Micrococcus luteus
tetrahydrofuran
inhibitory at concentrations above 2 M
Micrococcus luteus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
undecaprenyl diphosphate
-
Micrococcus luteus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Micrococcus luteus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for added metal ions
Micrococcus luteus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
undecaprenyl diphosphate + H2O
Micrococcus luteus
involved in cyclic peptidoglycan pathway
undecaprenyl phosphate + phosphate
-
Micrococcus luteus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micrococcus luteus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Micrococcus luteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
undecaprenyl diphosphate + H2O
-
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
undecaprenyl diphosphate + H2O
involved in cyclic peptidoglycan pathway
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-20
-
significant loss of activity by freezing and thawing one time
Micrococcus luteus
4
-
30-40% loss of activity within 1 week
Micrococcus luteus
25
-
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures
Micrococcus luteus
40
-
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures
Micrococcus luteus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.5
-
Micrococcus luteus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
-
Micrococcus luteus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethanol
slight activation at concentration of 4 M
Micrococcus luteus
methanol
slight activation at concentration of 4 M
Micrococcus luteus
additional information
significant rise in activity at high ionic strength
Micrococcus luteus
n-Propanol
activating at concentrations below 1.5 M
Micrococcus luteus
Triton X-100
-
Micrococcus luteus
Triton X-114
-
Micrococcus luteus
General Stability (protein specific)
General Stability
Organism
ascorbic acid stabilizes
Micrococcus luteus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
DTNB
slight inhibition
Micrococcus luteus
n-butanol
-
Micrococcus luteus
n-Propanol
inhibitory at concentrations above 1.5 M
Micrococcus luteus
p-chloromercuriphenylsulfonic acid
-
Micrococcus luteus
tetrahydrofuran
inhibitory at concentrations above 2 M
Micrococcus luteus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
undecaprenyl diphosphate
-
Micrococcus luteus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Micrococcus luteus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for added metal ions
Micrococcus luteus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
undecaprenyl diphosphate + H2O
Micrococcus luteus
involved in cyclic peptidoglycan pathway
undecaprenyl phosphate + phosphate
-
Micrococcus luteus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Micrococcus luteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
undecaprenyl diphosphate + H2O
-
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
undecaprenyl diphosphate + H2O
involved in cyclic peptidoglycan pathway
210607
Micrococcus luteus
undecaprenyl phosphate + phosphate
-
210607
Micrococcus luteus
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-20
-
significant loss of activity by freezing and thawing one time
Micrococcus luteus
4
-
30-40% loss of activity within 1 week
Micrococcus luteus
25
-
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures
Micrococcus luteus
40
-
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures
Micrococcus luteus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.5
-
Micrococcus luteus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
-
Micrococcus luteus
Other publictions for EC 3.6.1.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734263
Chang
Proposed carrier lipid-binding ...
Escherichia coli
J. Biol. Chem.
289
18719-18735
2014
-
-
1
-
17
-
2
1
-
2
1
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
17
-
1
2
1
1
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
734038
Shaaly
Undecaprenyl pyrophosphate pho ...
Enterococcus faecalis
J. Antimicrob. Chemother.
68
1583-1593
2013
-
-
-
-
-
-
-
-
-
-
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3
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1
1
1
1
-
-
718534
Coker
-
Current understanding of de no ...
Bacillus subtilis, Escherichia coli
Afr. J. Microbiol. Res.
5
2555-2565
2011
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-
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2
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2
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2
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2
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2
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-
-
-
-
-
-
2
2
-
-
-
718588
Hashizume
Tripropeptin C blocks the lipi ...
Micrococcus luteus
Antimicrob. Agents Chemother.
55
3821-3828
2011
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
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1
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1
2
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1
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-
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713052
Hynninen
An efflux transporter PbrA and ...
Cupriavidus metallidurans, Cupriavidus metallidurans CH34
Mol. Microbiol.
74
384-394
2009
-
-
1
-
-
-
2
-
1
-
-
-
-
9
-
-
1
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
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-
2
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1
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1
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12
-
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-
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-
-
-
-
687718
Touze
Substrate specificity and memb ...
Escherichia coli
J. Biol. Chem.
283
16573-16583
2008
4
-
1
-
-
-
-
6
3
-
3
1
-
2
-
-
1
-
-
-
5
-
8
2
1
-
-
6
1
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
6
3
-
3
1
-
-
-
1
-
-
5
-
8
2
1
-
-
6
1
1
-
-
-
-
-
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688861
Tatar
An Escherichia coli undecapren ...
Escherichia coli
Microbiology
153
2518-2529
2007
-
-
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1
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2
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1
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2
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1
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2
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1
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2
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1
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1
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2
-
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669336
El Ghachi
Identification of multiple gen ...
Escherichia coli
J. Biol. Chem.
280
18689-18695
2005
-
-
-
-
-
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2
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1
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1
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669366
Bernard
BcrC from Bacillus subtilis ac ...
Bacillus subtilis
J. Biol. Chem.
280
28852-28857
2005
-
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-
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2
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1
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4
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3
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2
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1
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3
-
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-
-
-
-
-
-
-
-
210607
Goldman
Purification and properties of ...
Micrococcus luteus
J. Biol. Chem.
247
5116-5122
1972
6
-
-
-
-
1
5
1
1
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
4
-
1
1
-
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6
-
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1
-
5
-
1
1
1
-
1
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1
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-
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2
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4
-
1
1
-
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-
-
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210606
Stone
Mechanism of action of bacitra ...
Enterococcus faecalis
Proc. Natl. Acad. Sci. USA
68
3223-3227
1971
-
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1
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1
9
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1
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1
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2
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1
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1
9
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1
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2
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