Cloned (Comment) | Organism |
---|---|
the expression of N-terminal His(6)-tagged Pwo dUTPase is performed in Escherichia coli BL21(DE3)pLysS and Eschwerichia coli Rosetta(DE3)pLysS strain that contains plasmid encoding additional copies of rare Escherichia coli tRNAs. Escherichia coli Rosetta(pLysS) strain is found with two times higher expression yield of His(6)-tagged Pwo dUTPase than Escherichia coli BL21(DE3)pLysS. The His(6)-tagged Pwo dUTPase is purified on Ni(2+)-IDA-Sepharose, dialyzed, and the enzyme activity is investigated. THe His(6)-tag domain has no influence on dUTP hydrolytic activity. dUTP is generated during PCR from dCTP, which inhibits the polymerization of DNA catalyzed by DNA polymerase with 3'-5' exonuclease activity | Pyrococcus woesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22043 | - |
x * 22043, His6-tagged enzyme, calculated from sequence | Pyrococcus woesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | Q6T180 | - |
- |
Pyrococcus woesei DSM 3773 | Q6T180 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus woesei |
Subunits | Comment | Organism |
---|---|---|
? | x * 22043, His6-tagged enzyme, calculated from sequence | Pyrococcus woesei |
Synonyms | Comment | Organism |
---|---|---|
dUTPase | - |
Pyrococcus woesei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus woesei | calculated from sequence, pI: 9.07 | - |
9.1 |