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Literature summary for 3.6.1.23 extracted from

  • Takacs, E.; Nagy, G.; Leveles, I.; Harmat, V.; Lopata, A.; Toth, J.; Vertessy, B.G.
    Direct contacts between conserved motifs of different subunits provide major contribution to active site organization in human and mycobacterial dUTPases (2010), FEBS Lett., 584, 3047-3054.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes D28N and T138STOP Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D28N/H145W the Asp/Asn mutation within Motif I results in approximately 50fold decrease of steady-state rate and significantly weakens the interaction of the protein with alpha,beta-imido-dUTP (15fold) whereas dUMP binding is only slightly affected Mycobacterium tuberculosis
D49N/F158W the Asp/Asn mutation within motif I results in approximately 1000fold decrease of steady-state rate and significantly weakens the interaction of the protein with alpha,beta-imido-dUTP (4fold) whereas dUMP binding is only slightly affected Homo sapiens
F158W the mutation has no significant effect on the catalytic properties of dUTPase Homo sapiens
H145W the mutation has no significant effect on the catalytic properties of dUTPase Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
alpha,beta-imido-dUTP
-
 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for full activity, In the absence of divalent cations, dUTPase retains about 50% of the catalytic rate observed in the presence of Mg2+ Homo sapiens
Mg2+ required for full activity, In the absence of divalent cations, dUTPase retains about 50% of the catalytic rate observed in the presence of Mg2+ Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mycobacterium tuberculosis P9WNS5
-
-
Mycobacterium tuberculosis H37Rv P9WNS5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Homo sapiens dUMP + diphosphate
-
 ?
dUTP + H2O
-
 Mycobacterium tuberculosis dUMP + diphosphate
-
 ?
dUTP + H2O
-
 Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
homotrimer
-
 Homo sapiens
homotrimer
-
 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
dUTPase
-
 Homo sapiens
dUTPase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 dUTP mutant enzyme D49N/F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
0.024
-
 dUTP mutant enzyme D28N/H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis
1.2
-
 dUTP mutant enzyme H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis
6.3
-
 dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens