Cloned (Comment) | Organism |
---|---|
gene dut, DNA and amino acid sequence determination and analysis, high level overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus mutans |
genes yncF and yosS, DNA and amino acid sequence determination and analysis, high level overexpression of N-terminally His6-tagged enzymes in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, 15 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 0.2 M MgCl2, 25% w/v PEG 3350, 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 1.3 A resolution | Streptococcus mutans |
purified recombinant selenomethionine-labelled YosS and YncF, hanging drop vapour diffusion method, for YosS: 30 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 2.0 M ammonium sulfate, 0.2 M potassium/sodium tartrate, 0.1 M trisodium citrate, pH 5.6, in 3 days, for YncF: 20 mg/ml protein in 20 mM Tris-HCl, and 150 mM NaCl, pH 7.5, with 0.2 M trimethylamine N-oxide dehydrate, 15% w/v PEG MME 2000, 0.1 M Tris-HCl, pH 9.0, in two weeks, X-ray diffraction structure determination and analysis at 2.3 A and 1.7 A resolution, respectively | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16100 | - |
3 * 16100, SDS-PAGE | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
genes yncF and yosS | - |
Streptococcus mutans | - |
gene DUT | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and gel filtration | Bacillus subtilis |
recombinant N-terminally His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and gel filtration | Streptococcus mutans |
Subunits | Comment | Organism |
---|---|---|
homotrimer | - |
Bacillus subtilis |
homotrimer | 3 * 16100, SDS-PAGE | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
dUTPase | - |
Bacillus subtilis |
dUTPase | - |
Streptococcus mutans |
YncF | - |
Bacillus subtilis |
YosS | - |
Bacillus subtilis |