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Literature summary for 3.6.1.23 extracted from

  • Vertessy, B.G.; Toth, J.
    Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases (2009), Acc. Chem. Res., 42, 97-106.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development dUTPase family of enzymes are promising targets for anticancer and antimicrobial therapies Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, phosphate chain coordination involves Mg2+, binding structure, overview. Mg2+ probably dissociates from the enzyme with the product PPi and not with dUMP Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Homo sapiens dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview dUMP + diphosphate dUMP is required for de novo biosynthesis of thymine ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P33316
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Reaction

Reaction Comment Organism Reaction ID
dUTP + H2O = dUMP + diphosphate catalytic reaction mechanism, overview Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell dUTPase is upregulated in human tumor cells Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview Homo sapiens dUMP + diphosphate dUMP is required for de novo biosynthesis of thymine ?
dUTP + H2O catalysis proceeds by way of an SN2 mechanism, a water molecule initiates in-line nucleophilic attack, conformational changes during the reaction take place in the C-terminus. The dUTPase binding pocket is highly specific for uracil, structural basis, overview. Phosphate chain coordination involves Mg2+ and is analogous to that of DNA polymerases. Catalytically competent and noncompetent conformation of the bound substrate, nodelling, overview Homo sapiens dUMP + diphosphate
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?

Subunits

Subunits Comment Organism
homotrimer folding pattern and active site architecture, overview Homo sapiens

Synonyms

Synonyms Comment Organism
dUTPase
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Homo sapiens
More the enzyme belongs to the dUTPase enzyme family Homo sapiens