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Literature summary for 3.6.1.23 extracted from

  • Varga, B.; Barabas, O.; Kovari, J.; Toth, J.; Hunyadi-Gulyas, E.; Klement, E.; Medzihradszky, K.F.; Toelgyesi, F.; Fidy, J.; Vertessy, B.G.
    Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase (2007), FEBS Lett., 581, 4783-4788.
    View publication on PubMed
Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged wild-type dUTPase and mutant F158W Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with inhibitor alpha,beta-imino-dUTP and Mg2+, 0.282 mM dUTPase, 1.25 mM alpha,beta-imino-dUTP, and 10 mM MgCl2 in 10 mM Tris-HCl, 50 mM NaCl, and 0.1 mM TCEP, pH 7.0, are mixed with reservoir solution containing 0.1 M Na-HEPES buffer, pH 7.5, and 18-20% PEG 3350, generation of rhombohedral crystals, X-ray diffraction structure determination and analysis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3'-deoxy-5'-O-(hydroxy{[hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl)uridine
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Homo sapiens dUTPase is essential for DNA integrity and is an important survival factor for cancer cells dUMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P33316
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type dUTPase and mutant F158W by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Homo sapiens dUMP + diphosphate
-
 ?
dUTP + H2O dUTPase is essential for DNA integrity and is an important survival factor for cancer cells Homo sapiens dUMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
dUTPase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61.7
-
 melting temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 dUTP recombinant wild-type and mutant enzymes Homo sapiens