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Literature summary for 3.6.1.23 extracted from

  • Bajaj, M.; Moriyama, H.
    Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana (2007), Acta Crystallogr. Sect. F, 63, 409-411.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, 25°C, 0.001 ml of protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.4, is mixed with 0.001 ml of reservoir solution containing 2 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.2-2.3 A resolution, molecular replacement Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Arabidopsis thaliana the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis dUMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9STG6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from to homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
Arabidopsis thaliana dUMP + diphosphate
-
?
dUTP + H2O the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis Arabidopsis thaliana dUMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
deoxyuridine triphosphate nucleotidohydrolase
-
Arabidopsis thaliana
dUTPase
-
Arabidopsis thaliana