Literature summary for 3.6.1.23 extracted from

Hidalgo-Zarco, F.; Camacho, A.G.; Bernier-Villamor, V.; Nord, J.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Leishmania major (2001), Protein Sci., 10, 1426-1433.

Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Leishmania major

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha-beta-imido-dUTP	competitive, strong inhibition	Leishmania major
dUDP	competitive	Leishmania major
dUMP	competitive	Leishmania major
additional information	diphosphate has no effect on activity	Leishmania major

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0021	-	dUTP	pH 8, 25°C	Leishmania major
0.0627	-	dUDP	pH 8, 25°C	Leishmania major

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	can partially replace Mg2+, causes increase in Km	Leishmania major
Mg2+	required, no activity in the absence of Mg2+	Leishmania major
Mn2+	can partially replace Mg2+, causes increase in Km	Leishmania major

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30300	-	2 * 30300, calculated from amino acid sequence	Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Leishmania major	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Leishmania major

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dATP + H2O	low activity	Leishmania major	dAMP + diphosphate	-	?
dCTP + H2O	low activity	Leishmania major	dCMP + diphosphate	-	?
dGTP + H2O	low activity	Leishmania major	dGMP + diphosphate	-	?
dTTP + H2O	low activity	Leishmania major	dTMP + diphosphate	-	?
dUDP + H2O	-	Leishmania major	dUMP + phosphate	-	?
dUTP + H2O	-	Leishmania major	dUMP + diphosphate	-	?
dUTP + H2O	removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival	Leishmania major	dUMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 30300, calculated from amino acid sequence	Leishmania major

Synonyms

Synonyms	Comment	Organism
dUTPase-dUDPase	hydrolyzes dUTP and dUDP	Leishmania major

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Leishmania major

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
4	60	very low activity above 60°C	Leishmania major

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	55	stable against denaturation	Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.4	-	dATP	pH 8, 25°C	Leishmania major
6.4	-	dGTP	pH 8, 25°C	Leishmania major
8.6	-	dTTP	pH 8, 25°C	Leishmania major
13	-	dCTP	pH 8, 25°C	Leishmania major
49	-	dUTP	pH 8, 25°C	Leishmania major
65	-	dUDP	pH 8, 25°C	Leishmania major

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	10	-	Leishmania major

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	10	strong increase of activity between pH 7 and 8	Leishmania major

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0009	-	alpha-beta-imido-dUTP	pH 8, 25°C	Leishmania major

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Release 2023.1 (January 2023)